Escherichia cloned gene expression

Robertus J, Piatak M, Ferris R, Houston L. Crystallization of ricin A chain obtained from a cloned gene expressed in Escherichia coli. J Biol Chem. 1987 262 19-20. 

Iwaki H, Y Hosegawa, S Wang, MM Kayser, PCK Lau (2002) Cloning and characterization of a gene cluster involved in cyclopentanol metabolism in Comamonas sp. strain NCIMB 9872 and biotransformations effected by Escherichia co/f-expressed cyclopentanone 1,2-monooxygenase. Appl Environ Microbiol 68 5671-5684. 

Mondello FJ (1989) Cloning and expression in Escherichia coli of Pseudomonas strain LB400 genes encoding polychlorinated biphenyl degradation. J Bacteriol 171 1725-1732. 

Oldfield s work confirmed the complete pathway and identified all the intermediates via an exhaustive experimental scheme, which included whole cell assays, cell-free extracts of IGTS8 as well as extracts from clones containing individual genes expressed in Escherichia coli. The need for NADH was clearly demonstrated in cell-free assays by amendment of NADH. The experimental evidence for involvement of FMN in the pathway was demonstrated [53,66,67],... 

R. Frothingham, W. A. Meeker-0 Connell, E. A. S. Talbot, J. W. George, K. N. Kreu-zer, Identification, Cloning, and Expression of the Escherichia coli Pyrazinamidase and Nicotinamidase Gene, pncA , Antimicrob. Agent Chemother. 1996, 40, 1426-1431. 

Amann, E., Brosius, J., and Ptashne, M. (1983). Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25, 167-178. 

Cary, J.W. Petersen, D.J. Papoutsakis, E.T. Bennett, G.N. Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli. J. Bacteriol., 170, 4613-4618 (1988)... 

Figure 5. Flavobacterium ATCC 39723 pentachlorophenol pathway genes expressed in Escherichia coli. Brackets indicate genes cloned into and functional in a recombinant E. coli.

Nakamura, K., Iwasaki, Y. Hattori, T. (1980). An improved Escherichia coli expression vector for the construction and identification of full-length cDNA clones. Gene 44, 347-51. 

A. F. Worrell and B. A. Connolly. Hie chemical synthesis of a gene coding for bovine pancreatic DNase I and Us cloning and expression in Escherichia coll. J, BioL Chem. 

Lee, P. and Swaisgood, H.E. 1998. Cloning and expression of a streptavidin-lipase fusion gene in Escherichia coli and characterization of the immobilized fusion protein. Enzyme Microb. Technol. 22, 246-254. 

Before the advent of genetic engineering, hGH was purified from cadavers pituitary glands, with several drawbacks. It was Genentech, the first biotech company founded in 1976, to clone and express the hGH gene in Escherichia coli, opening the way for the production of biopharmaceuticals through recombinant... 

Leung, P., Lee, Y. M., Greenberg, E., Esch, K., Boylan, S., and Preiss, J. 1986. Cloning and expression of the Escherichia coli gig C gene from a mutant containing an ADPglucose pyrophosphorylase with altered allosteric properties. J. Bacteriol. 167, 82-88. 

Shirai, T., Yamaguchi, H., Ito, H., Todd, C. W., and Wallace, R. B. (1985). Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor. Nature 313, 803-806. 

Kim, S.H. and Oriel, P. 2000. Cloning and expression of the nitrile hydratase and amidase genes from Bacillus sp. BR449 into Escherichia coli. Enzyme Microbiology and Technology, 27 492-501. 

Scrutton, N. S., Packman, L. C., Mathews, F. S., Rohlfs, R. J., and Hille, R., 1994, Assembly of redox centers in the trimethylamine dehydrogenase of bacterium WjA,. Properties of the wild-type enzyme and a C30A mutant expressed from a cloned gene in Escherichia coli, J. Biol. Chem. 269 13942913950. 

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