Equipment plate-based systems

Employing the conditions defined in the three data bases and the appropriate equations derived from the Plate and Rate Theories the physical properties of the column and column packing can be determined and the correct operating conditions identified. The precise column length and particle diameter that will achieve the necessary resolution and provide the analysis in the minimum time can be calculated. It should again be emphasized that, the specifications will be such, that for the specific separation carried out, on the phase system selected and the equipment available, the minimum analysis time will be absolute No other column is possible that will allow the analysis to be carried out in less time. 

High-throughput measurements are possible with commercially available HTS-FTIR systems. The analysis can be performed in a Tensor 27 FTIR spectrometer coupled to a HTS-XT system that can analyze samples on 96- or 3 84-well microtiter plates. The plates are equipped with a silicon plate for IR transmittance. Moreover, the ee values can be measured in culture supernatants, which is not possible with MS- or NMR-based assays (Sections 9.3.1 and 9.3.2). 

Production equipment that cannot be sterilized must be sanitized and disinfected by an appropriate method. This can be done by use of biocides like alcohols (70%), hydrogen peroxide, or formaldehyde-based chemicals or a combination of these. These can either be used for surface disinfections by wiping or spraying or even better by use of gas or dry fog systems for application of the disinfectants. The effect of cleaning and sanitation should be monitored. Microbiological media contact plates can be used to test critical surfaces, as inside the hot cells or glove boxes. The test samples must then be handled and monitored as radioactive contaminated units. 

The base (7) (6 x 7.4 x 1.5 cm) includes two reservoirs filled with the solvent system, one of which (5) is for development, the other (2), in the central part, being for chamber saturation if required. For development, the prepared TLC plate (6) with the coating facing downwards is placed on the central reservoir. The glass plate (4) (1x5 cm) in the left-hand solvent system reservoir is tilted (5) to cause development of the TLC plate to commence. The anti-evaporation hood (7) with a transparent top is placed over the equipment after the start of the process. 

---