Epitope analysis

Geysen, H M,Rodda, S J, Mason, T J., Tnbbick, G., and Schoofs, P. G (1987) Strategies for epitope analysis using peptide synthesis J Immunol Methods 102, 259-274... 

Frank, R. and Overwin, H. (1996) SPOT synthesis. Epitope analysis with arrays of synthetic peptides prepared on cellulose membranes. In Epitope Mapping Protocols, ed. G.E. Morris, pp. 149-169. Totowa, NJ Humana Press. 

Neudecker, P., Lehmann, K., Nerkamp, J., Haase, T., Wangorsch, A., Fotisch, K. et al. 2003. Mutational epitope analysis of Pru av 1 and Api g 1, the major allergens of cherry (Prunus avium) and celery (Apium graveolens) correlating IgE reactivity with three-dimensional structure. Biochem J 376(Pt 1) 97—107. 

Kahlert, H., Petersen, A., Becker, W.M., Schlaak, M. 1992. Epitope analysis of the allergen ovalbumin (Gal d II) with monoclonal antibodies and patients IgE. Mol Immunol 29 1191-1201. 

Skerritt, J.H., Hill, A.S., Andrews, J.L. 2000. Antigenicity of wheat prolamins Detailed epitope analysis using a panel of monoclonal antibodies. J Cereal Sci 32 259-279. 

Purification of antisera (10 ml) for mass spectrometric epitope analysis was per-formedon Protein A-Sepharose (Amersham Biosciences) according to manufacturer s instructions. Protein quantification was performed using the micro-BCA kit (Pierce, Rockford) positive elution fractions were concentrated to 7 (ig/ml. 

Emini, E. A., Jameson, B. A., Lewis, A. J., Larsen, G. R., and Wimmer, E. (1982). Poliovirus neutralization epitopes Analysis and localization with neutralizing monoclonal antibodies. J. Virol. 43, 997-1005. 

N. Venkatesh, S. Krishnaswamy, S. Meuris, G. S. Murthy. Epitope analysis and molecular modeling reveal the topography of the C-terminal peptide of the beta-subunit of human chorionic gonadotropin. Eur J Biochem. 1999, 265, 1061-1066. 

Y.M. van der Geld, et al., (2001). Antineutrophil cytoplasmic antibodies to proteinase 3 in Wegener s granulomatosis Epitope analysis using synthetic peptides. Kidney Int. 59, 147-159. 

M. Mahler, M. Bluthner and K. M. Pollard, (2003). Advances in B-cell epitope analysis of autoantigens in connective tissue diseases. Clin. Immunol. 107, 65-79. 

HM Geysen, SJ Rodda, TJ Mason, G Tribbick, PG Schoofs. Strategies for epitope analysis using peptide synthesis. J Immunol Meth 102 259-274, 1987. 

Yoshioka H, Ohmoto T, Urisn A, Mine Y, Adachi T (2004). Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat, i. Plant Physiol, 161 761-161. 

Wangorsch A, Ballmer-Weber BK, Rosch P, Holzhauser T, Vieths S (2007). Mutational epitope analysis and cross-reactivity of two isoforms of Api g 1, the major celery allergen. Mol Immunol, 44 2518-2527. 

Glostrup, Denmark) for use as an immunogen and standard. The second whole CRP protein, termed recombinant CRP (rCRP), was purchased from Oriental Yeast (Tokyo, Japan). This was used in the screening of hybridoma cells for the production of monoclonal antibodies. Five CRP fragments were prepared by the production of recombinant CRP fragments a method described below for epitope analysis. 

The results of epitope analysis using Western blot are presented in Table 2. MoAbs 4, 7, and 8 were found to react with all fragments, while MoAb 5 reacted with CRP-01, CRP-02, CRP-... 

Rabjohn P, Helm EM, Stanley IS, West CM, Sampson HA, Burks AW, Bannon GA. Molecular cloning and epitope analysis of the peanut allergen, Ara h 3. J Clin Invest 1999 103 535-542. 

---