Enzyme reuse

Enzymatic Reactors Adding free enzyme to a batch reactor is practical only when the value of the enzyme is relatively low. With expensive enzymes, reuse by retaining the enzyme with some type of support makes great economic sense. As some activity is usually lost in tethering the enzyme and the additional operations cost money, stabihty is very important. However, many enzymes are stabilized by immobilization thus, many reuses may be possible. 

It has also been suggested that solubihzation of enzymes in organogels allows interesting reaction media to be realized since they facilitate enzyme reuse and easy product separation [281],... 

The possibility of immobilized enzyme reuse was confirmed for monolaurin synthesis. This aspect, not often investigated, certainly increases the economical viability of the process. 

From Table 1 and Fig. 7, there are several trends worth noting. First, using the lower enzyme concentration of 10 FPU/g of cellulose is economical only at high enzyme costs. Second, when the enzyme price declines below about 10reduced enzyme use. Third, enzyme reuse by employing the semibatch process is economically attractive only for... 

Another advantage of this enzyme-catalyzed route to colloidal PAn salts is the considerably higher pH that can be employed compared to the previous chemical and electrochemical polymerization methods. Horseradish peroxidase immobilized on chitosan powder as a solid support has also been found to catalyze the H202 oxidation of aniline to a similar PAn/PSS product, opening up the prospect of enzyme reuse and the design of enzyme reactors for PAn synthesis.104... 

Besides the classical engineering question of reactor choice, the most important point in enzyme reactor design is the aspect of enzyme reuse, either by immobilization or by separation from the product stream. Batch processes without enzyme reuse are only possible if the costs of the biocatalyst are negligible. Different reactor techniques addressing the aspect of enzyme reuse are discussed in the following sections. 

The use of membrane reactors for enzyme-catalyzed bioremediation processes is potentially beneficial in terms of improving the process economics by enabling enzyme reuse and enhancing overall efficiency and robustness. On one hand, various enzymes can efficiently degrade wide varieties of emerging pollutants. On the other hand, membrane reactors can eliminate... 

Y.L., and Shieh, C.J. (2011) Continuous production of lipase-catalyzed biodiesel in a packed-bed reactor optimization and enzyme reuse study. J. Biotned. Biotechnol., Article ID 950725, 6 pp. doi 10.1155/2011/950725... 

Stamatis, H., Xenakis, A., Kolisis, F.N. 1995. Studies on enzyme reuse and product recovery in Upase-catalyzed reactions in microemulsions. Ann. NY Acad. Sci. 750, 237-241. 

Larsson, K.M., Adlercreutz, R, Mattiasson, B. 1990. Enzymatic catalysis in microemulsions Enzyme reuse and product recovery. Biotechnol. Bioeng. 36,135-141. 

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