Enzyme immunoassay receptor

M22. Mobbs, B. G., lohnson, I. E., and Lui, Y., Quantitation of cytosolic and nuclear estrogen and progesterone receptor in benign, untreated, and malignant human prostatic tissue by radioligand binding and enzyme-immunoassays. Prostate 16, 235-244 (1990). 

An extension of enzyme immunoassay is the enzyme affinity assay applicable to studies of nonimmunological interactions. This is already exemplified by the measurement of hormone using its receptor and by our studies on the interaction of fibronectin with collagen. - Assays of these and similar principles might well become a new area of expression for EIA. 

Titration assays, enzyme immunoassays, and immuno-cytochemical assays are used to measure steroid hormone receptors. 

The classic quantitative biochemical method for assaying steroid receptors in tumor tissue specimens is the multiple-point dextran-coated charcoal (DCC) titration assay. However, in comparison with the classic DCC assays, enzyme immunoassays are preferred as they cost less and are simpler, require less time, and can be performed using less tissue than DCC titration assays. 

Cavaliere A, BucciareUi E, Sidoni A, Bianchi G, Pietropaoli N, et al. Estrogen and progesterone receptors in breast cancer Comparison between enzyme immunoassay and computer-assisted image analysis of immunocytochemical assay. Cytometry 1996 26 204-08. 

Hohnes FA, Fritsche HA, Loewy JW, Geitner AM, Sutton RC, et al. Measurement of estrogen and progesterone receptors in human breast tumors Enzyme immunoassay versus binding assay. J Clin Oncol 1990 8 1025-35. 

Dowsett M. Comparison of new immunohisto-chemical assay for estrogen receptor in paraffin wax embedded breast carcinoma tissue with quantitative enzyme immunoassay. J Cfin Pathol 1994 47 900-5. 

Non-competitive enzyme immunoassays with antibodies or receptor molecules immobilized on the solid phase... 

TA Aasmundstad, et al. Estrogen receptor analysis—Correlation between enzyme immunoassay and immunohistochemical methods. J Clin Pathol 45 125, 1992. 

GR Adolf, et al. A monoclonal antibody based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein-I, a soluble fragment of the 60 KDa TNF receptor, in biological fluids. J Immunol Meth 143 127, 1991. 

D22. Dittmer, R., Harfmann, P., Tenschert, W., Arndt, R., and Busch, B. Monitoring of renal transplant patients with interleukin-2 and interleukin-2 receptor enzyme immunoassay and interleukin-2 receptor immunocytology. Transplant. Proc. 22, 2284-2285 (1990). 

Menendez-Botet, C. J., Stankievic, R., Schwartz, D., and Schwartz, M. K., Comparison of the quantitative measurement of human estrogen and progesterone receptor in tissue cytosol by the dextran-coated charcoal assay and the Abbott monoclonal enzyme immunoassay. Clin. Chem. (Winston-Salem, N.C.) 34(6), 1225 (1988). 

Wl. Weigand, R. A., Cotter, D. L., Dunn, R. A., Nolan, C., Greene, G., and Przywara, L. W., Quantitation of progesterone receptor (PgR) in human breast tumors by double monoclonal enzyme immunoassay. Breast Cancer Res. Treat. 8(1), 87 (1976). 

The FPIA method can be potentially used either independently or as a complementary method to enzyme-linked immunosorbent assay (ELISA) and fluorescence polarization immunoassays (FPIA) technique. FCIA does not need polarization equipment and is not markedly influenced by light scattering effects. The FCIA technique can be expanded for analysis of enzymes and receptors including adaptation to fibro-optic techniques. 

There should be specific, saturable binding to the receptor, accompanied by pharmacological characteristics appropriate to the functional effects, demonstrable using a radioactive, eg, tritium or iodine-125, ligand to label the receptor. Radioligand binding assays (1,6) have become a significant means by which to identify and characterize receptors and enzymes (see Immunoassays Radioactive tracers). Isolation of the receptor or expression of the receptor in another cell, eg, an oocyte can be used to confirm the existence of a discrete entity. 

---