Enterococcus biofilms

The probiotic strain E. faecium PR88 was studied in clinical trial (Allen et al. 1996). The consumption of this strain led to alleviation of the symptoms of irritable bowel syndrome in humans. The efficacy and safety of live combmedBifidobacterium, Lactobacillus and Enterococcus capsules in treatment of irritable bowel syndrome was also demonstrated (Fan et al. 2006). E. faecium functions as a probiotic strain inhibiting biofilm formation by Streptococcus mutants, which is an etiological agent for dental caries (Kumada et al. 2008). 

Kumada M, Senpuku H, Motegi M, Nkao R, Yonezawa H, Yamamura H, Watanabe H, Tagami J (2008) Effects of Enterococcus faecium on Streptococcus mutans biofilm formation using flow ceU system. J Oral Biosci 50 68-76... 

Minei CC, Gomes BC, Ratti RP, D Angehs GEM, De Martinis ECP (2008) Influence of peroxyacetic acid and nisin and coculture with Enterococcus faecium on Listeria monocytogenes biofilm formation. J Eood Prot 71(3) 634-638... 

Giardino, L., Ambu, E., Savoldi, E., Rimondini, R., CassaneUi, C., Debbia, E.A., 2007. Comparative evaluation of antimicrobial efficacy of sodium hypochlOTite, MTAD, and Tetraclean against Enterococcus faecaUs biofilm. Journal of Endodontics 33, 852—855. Gilani, G.S., Xiao, C., Lee, N., 2008. Need for accurate and standardized determination of amino acids and bioactive peptides for evaluating protein quality and potential health effects of foods and dietary supplements. Journal of AOAC International 91, 894—900. 

Living biofilms are not a strict prerequisite for adhesion prevention. Excreted compounds such as EPS or biosurfactants alone are often inhibiting. Purified biosurfactants from Lactobacillus casei, L. fermentum, and L. acidophilus were able to reduce the number of pathogenic Enterococcus faecalis to biosurfactant-coated glass by up to 77 % compared to uncoated controls [20]. Based on the method used for extracting and purifying biosurfactants, one can assume that bacterial EPS were indeed these... 

Nallapareddy, S.R., Singh, K.V., Sillanpaa, J., et al. (2006) Endocarditis and biofilm-associated pili of Enterococcus faecalis. J Clin Investig 116, 2799—2807. 

Cheese wooden Lactococcus sp., Leuconostoc sp., Enterococcus sp. Leuconostoc sp., Multispecies biofilms with food-borne pathogens B biofilms on wooden shelves maintain etal.2012) (Mariani et al. 2007)... 

Guiton, P.S., Hung, C.S., Kline, K.A., et al. (2009) Contribution of autolysin and Sortase A during Enterococcus faecalis DNA-dependent biofilm development. Infect Immun 77, 3626-3638. 

Hancock, L.E. and Perego, M. (2004) The Enterococcus faecalis fsr two-component system controls biofilm development through production of gelatinase. J Bacterial 186, 5629-5639. 

Mohamed, I.A., Huang, W., Nallapareddy, S.R., et al. (2004) Influence of origin of isolates, especially endocarditis isolates, and various genes on biofilm formation by Enterococcus faecalis. Lnfect Lmmun 72, 3658—3663. 

Theilacker, C., Sava, L, Sanchez-Carballo, R, et al. (2011) Deletion of the glycosyltransferase bgsB of Enterococcus faecalis leads to a complete loss of glycolipids from the cell membrane and to impaired biofilm formation. BMC... 

Socha, A.M., LaPIante, KL., Russell, D.J., and Rowley, D.C. (2009) Stmcture-activity studies of echinomydn antibiotics against dmg-resistant and biofilm-forming Staphylococcus aureus and Enterococcus faecalis. Bioorg. Med. Chem. Lett., 19,1504—1507. 

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