Endothelial Cell Surface Heparan Sulphate,

Also several serinprotease inhibitors will be adsorbed to surfaces. They may inactivate activated serinproteases via complex formation resulting in antithrombogenic surfaces. These reactions may be catalytically modulated by surface bound polysaccharides such as heparan sulphate, heparin, dermatan sulphate and covalently bound chondroitin sulphate in thrombomoduline . Most of the heparan sulphates on the luminal surface of blood vessels, in the glycocalix, have been reported to be AT III inactive. The endothelial cell surface heparan sulphate (ESHS) isolated by us is AT... 

ISOLATION AND CHARACTERISATION OF ENDOTHELIAL CELL SURFACE HEPARAN SULPHATE FROM WHOLE BOVINE LUNG FOR COATING OF BIOMATERIALS TO IMPROVE HAEMOCOMPATIBILITY... 

Isolation of Endothelial Cell Surface Heparan Sulphate... 

Scheme 1. Main isolation steps ofbovine lung endothelial cell surface heparan sulphate.

Figure 6. The left of the above shown spectra belongs to the described preparation while the right one is derived from aortic endothelial cell surface heparan sulphate from cell culture. The conformity between the two spectra again indicates the equality of the two substances. The degree of acetylation was determined to 52,6% in comparison to the cell culture derived material for which a degree of acetylation of 56,1% was measured.

In the present paper a new method for the isolation of bovine lung endothelial cell surface heparan sulphate is described. The aim of this work was to investigate for an alternative source leading to ES-HS as described from bovine aortic cell culture. Because of the excellent properties cell culture ES-HS coating of biomaterials has shown in in vitro as well as in "in vivo" experiments, a commercially available source should be found. The solution presented in this work leads to sufficient amounts for technical application of the coating system, especially the isolation procedure bears the potential of a high degree of automation for industrial purposes. 

EC-SOD is a copper-zinc enzyme located on endothelial cell surfaces. It is believed that EC-SOD binds to the vasculature via specific glycosaminoglycans — probably heparan sulphate on the endothelium. The association of EC-SOD with endothelial cell surfaces may indicate a cell-specific protective role. Eig/ity per cent of SOD activity in control, noninflamed synovial fluid is due to EC-SOD and its concentration is decreased by 50% in RA fluid (Marklund etal., 1986). 

In the near future further investigations will be carried out such as testing of cytotoxicity, different methods of sterilisation (steam, y, EO) and animal experiments to become a better insight to the potential of bovine lung ES-HS. All these investigations already have been carried out successfully for the cell culture derived material and according to the similarity between the two substances we are hopeful to gain the same results as for bovine aortic endothelial surface heparan sulphate. 

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