Embryoid bodies formation

Kurosawa H (2007) Methods for inducing embryoid body formation in vitro differentiation system of embryonic stem cells. J Biosci Bioeng 103(5) 389-398... [Pg.75]

Dang SM, Kyba M, Perlingeiro R, Daley GQ, Zandstra PW (2002) Efficiency of embryoid body formation and hematopoietic development from embryonic stem cells in different culture systems. Biotechnol Bioeng 78(4) 442 53... [Pg.76]

Koike M, Kurosawa H, Amano Y (2005) A round-bottom 96-well polystyrene plate coated with 2-methacryloyloxyethyl phos-phorylcholine as an effective tool for embryoid body formation. Cytotechnology 47(1-3) 3-10... [Pg.76]

Human Embryoid body formation 21 days (hypoxic or normoxic) Hypoxic (2% O2) or normoxic (20% O2), high density plating Growth factor free chondrogenic media 28 days Hypoxic conditions resulted in 3.4-fold increase in type II collagen and 1.9-fold increase in GAG production, 3-fold increase in tensile modulus... [Pg.604]

Murine Embryoid body formation 4 days Bilayered PEODA hydrogel with hepatic cells 10 ng/mL TGF-Pl 21 days Coculture 4-fold increase in GAG production, 80-fold increase in aggrecan mRNA expression. Type n collagen mRNA and staining only in coculture groups... [Pg.604]

Human Embryoid body formation 9 days Scaffolds Matrigel and 50/50 blend PLGA/PLLA 2 ng/mL TGF-pl, 10 ng/mL IGF-1 14 days Immunostaining showed cartilage-like tissue with GAG present... [Pg.604]

Nakagawa, T., S.Y. Lee, and A.H. Reddi, Induction of chondrogenesis from human embryonic stem cells without embryoid body formation by bone morphogenetic protein 7 and transforming growth factor hetM. Arthritis Rheum, 2009. 60(12) 3686-92. [Pg.616]

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