Embryo in situ hybridization

Akam Hafen et al Levine et al. Drosophila embryo in situ hybridization... 

The CK library membrane-associated embryonic cDNA clones are in general not full length, but have been analyzed by whole-embryo in situ hybridization. Data from this library can be queried for clones expressed in particular tissues. 

Stern CA. 1995. Detection of multiple gene products simultaneously by in situ hybridization histochemistry and immunohistochemistry in while mounts of avian embryos. Curr Top Dev Biol 36 223-243. 

Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos... 

Hume DA, Monkley SJ, Wainwright BJ. Detection of c-fms protooncogene in early mouse embryos by whole mount in situ hybridization indicates roles for macrophages in tissue remodelling. British Journal of Haematology 1995, 90, 939-942. 

Nonradioactive in Situ Hybridization Simplified Procedures for Use in Whole Mounts of Mouse and Chick Embryos... 

The in situ hybridization technique is used in several large-scale efforts to provide a comprehensive pictnre of the gene expression pattern in for example mouse embryos and brain (17). 

Rosen B, Beddington R (1993) Whole-mount in situ hybridization in the mouse embryo gene expression in three dimensions. Trends Genet 9 162-167... 

Singer and colleagues (68, 69) have successfully visualized cytoskeletal mRNAs and their associate proteins using biotinylated cDNA probes followed by antibodies to biotin and collodial gold-conjugated antibodies. Their method used in situ hybridization followed by whole mount TEM of Triton-extracted chicken embryo fibroblasts. Cytoskeletal mRNAs were found in close proximity to actin protein and further from tubulin filaments. While the whole mount technology does limit the technique to extracted cells, applications to thin sections will allow greater resolution. 

Peripheral axotomy caused a decrease of Y1 mRNA in the small neurons and an increase in large neurons. These results suggested that the DRG Y1 receptor is mosdy a prejunctional receptor in primary afferent neurons and that it may play a role in the modulation of somatosensory information (Zhang et al., 1994). Low levels of Y1 mRNA have also been reported for rat splenic lymphocytes as documented by a partial cDNA clone, PCR and RNase protection (Petitto et al., 1994). A developmental study by in situ hybridization of whole rat embryo sections (Jazin et al., 1993) detected the earliest Y1 mRNA around day 14, both in diencephalon and spinal cord. 

In Situ Hybridization Can Detect Transcription Changes in Intact Tissues and Permeabilized Embryos... 

A EXPERIMENTAL FIGURE 15-19 Maternally derived bicoid mRNA is localized to the anterior region of early Drosophila embryos. All embryos shown are positioned with anterior to the left and dorsal at the top. In this experiment, In situ hybridization with a radloactively labeled RNA probe specific for bicoid mRNA was performed on whole-embryo sections 2.5-3.5 hours after fertilization. 

Using in situ hybridization with a dorsal-specific probe, where in the syncytial Drosophila embryo would one expect to find dorsal expressed Using immunohistochemistry with an anti-dorsal antibody, where would you expect to find Dorsal protein expressed ... 

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