Ellipsometric microscopy

A newer and perhaps more useful application of ellipsometry to Langmuir films is their lateral characterization via ellipsometric microscopy [146], A simple modification of the nuU ellipsometer allows one to image features down to 10-/im resolution. Working with a fixed polarizer and analyzer, some domains are at extinction while others are not and appear bright. This approach requires no fluorescent label and can be applied to systems on reflective supports. 

Figure 17 Principle of ellipsometric microscopy. Full arrows symbolise the light path of illumination, broken arrows stand for the observation, respectively. A parallel beam of polarised light is focussed into an ofT-axis spot in the back focal plane of the lens. In the front focal plane of the lens, where the object is located, this results In a parallel polarised beam of light hitting the object under a shallow angle of incidence. Light reflected by the object is collected by the lens, passes a motorised polarization analyzer and is focused onto a digital CCD camera. For clarity, several optical elements are omitted, (redrawn from [36])...

Figure 18 Schematic view of the micropatterned Langmuir-Blodgett film with all relevant dimensions Ellipsometric microscopy images of the sample. The angle of incidence was ip = 55.8°. The gray scales from black to white represent a change in A of 120 - 130°. The scale bar has a length of 25/im. (redrawn from [36])...

Munteanu, S., N. Garraud, J. P. Roger, F. Amiot, J. Shi, Y. Chen, C. Combellas, and F. Kanoufi. In situ, real time monitoring of surface transformation Ellipsometric microscopy imaging of electrografting at microstructured gold surfaces. Anal. Chem. 85, 2013 1965-1971. 

Herron, J. N., Muller, W., Paudler, M., Riegler, H., Ringsdorf, H., Sici, P. A. (1992). Specific recognition-induced self-assembly of a biotin lipid/streptavidin/Fab fragment triple layer at the air/water interface Ellipsometric and fluorescence microscopy investigations, Langmuir, 8 1413. 

Structure (1-100 (itn). An acidreactive modification of the polymer blend film converted part of the organic template to a sUica network through exposure to precursor vapors. The impact of the polymer blend composition on the structure of the porous silica films was examined with the stmcture of the porous silica films determined using X-ray diffraction (XRD), SEM, transmission electron microscopy (TEM), spectroscopic ellipsometry (SE), and ellipsometric porosimetry (EP). 

The adsorption kinetics can be followed by ellipsometric measurements and the morphology of the film can be characterized by TEM coupled to a carbon platinum shadowed replica and atomic force microscopy (AFM) [14, 37]. For molecular concentration under the CMC,... 

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