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Phenol ELISA

New detection methods of phenolic compounds are being developed. Based on the principle of the enzyme-linked immunosorbent assay (ELISA), a method has been developed to quantify phenolic compounds such as isoflavones (Vergne and others 2007). [Pg.66]

N0O Donryu female rats ( 8 weeks old) were administered an intravenous injection of [- H]4HAQ0 at 20 mg per kg body weight. After one hour, the liver, pancreas, kidney, lung and uterus tissues were excised and DNA samples isolated by phenol extraction combined with protease and RNase A treatment. The amounts of ANQO adducts were determined by UV absorbance at 260 nm and by measurement of radioactivity. The DNA samples were denatured by heating for 5 min at 90°C, and were assayed by competitive ELISA. [Pg.250]

The need for maximum sample throughput and minimal human interaction within analytical procedures has provided considerable impetus to the development of integrated systems. SPE-LC in-tube SPME followed by ultraviolet (UV) or MS detection and membrane introduction mass spectrometry (MIMS) have both been used to this end. Submersible MIMS systems capable of extended underwater deployment down to 200 m and with a mass range of up to 200 amu have recently come onto the market. Elow injection coupled with MIMS allows fast, near-real-time determination of, for example, phenols in water. Derivatization of the phenols with acetic anhydride can be used to enhance both the selectivity and sensitivity of this method. Other online derivatization procedures are under development with a view to increasing the scope for rapid determination of highly polar compounds that have previously proved difficult to analyze. Large volume injection techniques and developments in enzyme-linked immunosorbent assay (ELISA) technologies... [Pg.5065]

Such a variety of compounds requires the application of the full panoply of analytical techniques including ELISA, LC, LC-MS, GC-MS (negative and positive ion), GC-MS-MS. Newer methods may include online SPE systems combined with MS or UV or electrochemical detection systems. One important consideration is that analysis of a particular EDC should also include its degradation and/or metabolic products because these too may remain active. So for alkyl phenols the concurrent measurement of the appropriate alkyl phenol ethoxylates and alkyl phenoxycarboxylic acids is environmentally important and a significant analytical challenge. [Pg.5069]

Due to the labor-intensive nature of monitoring programs, alternatives have been sought. Enzyme-linked immunosorbent assay (ELISA) has been proposed (Kuniyuki et al., 1984). The advantage of ELISA is that viable yeasts need not be present. Unfortunately, the method is too sensitive for routine production applications and, at present, too costly. The volatile phenol, 4-ethylphenol, has been proposed as a marker for present/past growth of Brettanomyces and Dekkera in wine. For details regarding analysis, see Zoecklein et al. (1995). [Pg.77]

Cytotoxicity studies. The MCT-7 breast cancer cells were plated at a density of 2 x 10 cells in 0.2 ml of DMEM on 96-well plates and incubated at 37°C in a 5% CO2 atmosphere. Thereafter, the medium in the weUs was replaced with 150 pi of the microemulsion systems and the incubation was continued overnight. The influence of microemulsions on MCF-7 cell survival was assayed after 4-h incubation at 37°C using MTT as it was described in [2]. The MTT was dissolved in PBS (5 mg/ml) and diluted 1 10 in medium without phenol red and serum. A volume of 1(X) pi of MTT was added to each well after removing the microemulsion from the wells. Then the cells were incubated for 2 h at 37°C to form insoluble formazan crystals and then dissolved in acidic iso-propanol. The absorbance of the resulting solutions from three duplicate experiments was read (at 570 nm) in an ELISA microplate reader (Labsystems Multiscan MS, Finland). The calculated data were expressed as the percentage of cell survival. [Pg.194]


See other pages where Phenol ELISA is mentioned: [Pg.27]    [Pg.149]    [Pg.158]    [Pg.161]    [Pg.162]    [Pg.164]    [Pg.164]    [Pg.166]    [Pg.166]    [Pg.29]    [Pg.19]    [Pg.659]    [Pg.71]    [Pg.562]    [Pg.310]    [Pg.1233]    [Pg.928]    [Pg.131]    [Pg.138]    [Pg.77]    [Pg.97]    [Pg.639]    [Pg.5068]    [Pg.381]   
See also in sourсe #XX -- [ Pg.359 ]




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