Electrospun polyurethane scaffolds in tissue engineering applications

5 Electrospun polyurethane scaffolds in tissue engineering applications 

A biodegradable poly (ester urethane)urea (PEUU) was blended with a phospholipid polymer of poly(methacryloyloxyethyl phosphorylcholine-co-methacryloyloxyethyl butyl urethane) (PMBU) (molar ratio=70/30), in HFIP solvent, and then coelectfospun 

A bilayer scaffold including an outer layer of an electrospun PEUU scaffold and an inner layer of porous PEUU scaffold was developed to address the cellular-ization issue [15]. The electrospun layer provided the mechanical support, while the porous scaffold layer supplied space for cell loading and infiltration. Prior to implantation, the cells including primary cells or stem cells were uniformly seeded into the porous layer using a customized vacuum device [15,40 2]. Rat vascular smooth muscle cells were seeded into the bilayer conduit and cultured in vitro for 2 days, and then this cellularized scaffold was implanted into the rat aorta model [42]. After 8 weeks, the patency of the cellularized scaffolds increased by 75% compared to 38% of the acellular scaffold. The failed scaffolds were blocked due to the intimal hyperplasia. The patent scaffolds contained a neointimal layer consisting of multiple layers of the immature contractile smooth muscle 

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