Electrophoresis continuous

In preparative continuous free flow electrophoresis, continuous buffer and sample feed are introduced at one end of a thin, rectangular electrophoresis chamber. A schematic is presented in Fig. 11-5. The sample stream is usually introduced through a single port while buffer is introduced through several ports, essentially producing a buffer curtain . Because the buffer streams are introduced independently, it is fairly easy to establish a variety of gradients (e.g., pH, density, ionic strength) across the buffer curtain . 

DGE a AC AMS APCI API AP-MALDI APPI ASAP BIRD c CAD CE CF CF-FAB Cl CID cw CZE Da DAPCI DART DC DE DESI DIOS DTIMS EC ECD El ELDI EM ESI ETD eV f FAB FAIMS FD FI FT FTICR two-dimensional gel electrophoresis atto, 10 18 alternating current accelerator mass spectrometry atmospheric pressure chemical ionization atmospheric pressure ionization atmospheric pressure matrix-assisted laser desorption/ionization atmospheric pressure photoionization atmospheric-pressure solids analysis probe blackbody infrared radiative dissociation centi, 10-2 collision-activated dissociation capillary electrophoresis continuous flow continuous flow fast atom bombardment chemical ionization collision-induced dissociation continuous wave capillary zone electrophoresis dalton desorption atmospheric pressure chemical ionization direct analysis in real time direct current delayed extraction desorption electrospray ionization desorption/ionization on silicon drift tube ion mobility spectrometry electrochromatography electron capture dissociation electron ionization electrospray-assisted laser desorption/ionization electron multiplier electrospray ionization electron transfer dissociation electron volt femto, 1CT15 fast atom bombardment field asymmetric waveform ion mobility spectrometry field desorption field ionization Fourier transform Fourier transform ion cyclotron resonance... 

In the simplest form of polyacrylamide gel electrophoresis (continuous zonal electrophoresis) the compositions of the electrode and gel buffers are the same separation is effected by differences in mass and charge, the latter can be altered by the choice of buffer. At pH 8-9 (for example, in diethylbarbiturate buffer) most pro-... 

Recovery of compounds by electrophoresis, continued until the sample migrates off the end of the supporting medium, can also be achieved for some forms of electrophoretogram, for example, preparative polyacrylamide rods, although this requires a special attachment at the base of the column to bleed off the sample as it emerges. 

HPLC, TLC, and ITLC analysis methods available today are more convenient and reproducible than electrophoresis. Despite this, electrophoresis continues to be used as a research tool in radiopharmaceutical studies. The reason for this is that electrophoresis gives information on the charge of " Tc complexes that caimot be easily obtained by other techniques. From the standpoint of determining whether a specific radiolabeled complex is anionic, cationic or neutral, electrophoresis is the best technique currently available. 

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