Rectangular electrophoresis chambers

In preparative continuous free flow electrophoresis, continuous buffer and sample feed are introduced at one end of a thin, rectangular electrophoresis chamber. A schematic is presented in Fig. 11-5. The sample stream is usually introduced through a single port while buffer is introduced through several ports, essentially producing a buffer curtain . Because the buffer streams are introduced independently, it is fairly easy to establish a variety of gradients (e.g., pH, density, ionic strength) across the buffer curtain . 

FIG. 4 The rectangular electrophoresis chamber for electrokinetic characterization of macroscopic surfaces. 

Consider now the general case of a rectangular electrophoresis chamber of width la and depth lb as shown in Figure 19.6. In such a chamber, the upper surface, lower surface and sidewalls may possess different electrokinetic properties. Solutions to equation (19.23) describing the fluid flow in the chamber in this case are subject to the following conditions ... 

In [139] a new design of an electrophoresis cell with a flat rectangular chamber is presented in which the smallest cell dimension is arranged parallel and not perpendicular to the electric field. It is claimed that this arrangement allows a simple scale-up. 

A 1-mol/L solution of potassium chloride was mixed with sufficient agarose (Electrophoresis Purity Reagent BioRad Laboratories, Richmond, CA) to give a 1.0% solution which was heated until the agarose dissolved. The acrylic chambers ( Fig. 3) to hold the gel were rectangular (16 mm in length, 8 mm wide, and 1.5 mm thick) with a cylindrical central depression (6 mm diameter and 1.5 mm depth). 

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