Continuous detection methods capillary electrophoresis

This section deals briefly with classical methods based on conventional mixing of the sample and reagents such as the batch mode and low-pressure flow mixing methods, as well as the use of CL detection in continuous separation techniques such as liquid chromatography and capillary electrophoresis for comparison with the unconventional mixing mode. 

Specifically, Chapter 2 discusses the concept of sample preparation and its implications. Ways of minimizing or avoiding the main problems posed by solid and liquid samples with the aid of US applied in the typical scenarios for two analytical chemical works viz. discrete and continuous systems) are proposed. Also, the use of US prior to sample preparation is discussed before dealing with specific sample preparation methods suited to the physical state of the sample and the treatment it required for presentation to continuous separation equipment (whether a chromatograph or a capillary electrophoresis module) or directly to the detector for monitoring, detection, characterization and (or) quantification. 

Capillary electrophoresis technology has become an indispensable tool for forensic scientists in the biology field since it is able to provide valuable information to aid in the process of law enforcement. The primary application of the technique is in the qualitative analysis of STRs. Isolation of STR mixtures is also possible using relative peak heights. Other applications of CE include quantitative analysis of PCR products, mtDNA sequencing, and mutation detection for the analysis of plant and bacterial DNA. Based on the performance of the methods illustrated above, it is reasonable to expect future researchers and practitioners to continue working to exploit the capabilities of this robust scientific technique and its application to criminal investigations. 

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