Pre-embedding electron microscopic

Burry, R. W., Vandre, D. D., and Hayes, D. M. (1992) Silver enhancement of gold antibody probes in pre-embedding electron microscopic immunocytochemistry. J. Histochem. Cytochem. 40, 1849-1856. 

There are two different ways to perform electron microscopic immunocytochemistry pre-embedding and postembedding (Stirling, 1990). Pre-embedding electron microscopic immunocytochemistry applies the antibodies and label to samples just after fixation but before embedding in epoxy resin and sectioning. Postembedding electron microscopic immunocytochemistry applies antibodies and label to thin sections made after the samples have been embedded in epoxy resin and sectioned. 

The dehydration, embedding, and sectioning of pre-embedding electron microscopic immunocytochemistry blocks follow the standard electron microscopic procedures. However, for section staining, the time is reduced to 2-3 min to preserve the size of the silver particles. 

Pre-embedding electron microscopic immunocytochemistry - a method that uses antibodies for incubation in the tissue before the embedding in epoxy resin. 

Silver enhancement - used in pre-embedding electron microscopic immunocytochemistry to enlarge small gold particles to a size seen in the electron microscope. 

Priestley JV, Alvarez FJ, Averill S (1992) Pre-embedding electron microscopic immunocytochemistry. In Polak JM, Priesdey JV (eds) Electron microscopic immunocytochemistry. Oxford University Press, Oxford, pp 89-121... 

Understanding the difference between processing for pre-embedding and postembedding electron microscopic immunocytochemistry provides a basis for selecting the better technique (Table 15.1). 

Table 15.1 Conditions for pre-embedding and postembedding electron microscopic immunocytochemistry...

Anderson KD, Karle EJ, Reiner A (1994) A pre-embedding triple-label electron microscopic immunohistochemical method as applied to the study of multiple inputs to defined tegmental nemons. J Histochem Cytochem 42 49-56... 

Combined neurotracing and immunolabeling can also be applied for electron microscopic analysis. We usually visualize BDA-labeled CFs with immunoperoxidase and DAB, and molecular markers with pre-embedding silver-enhanced immunogold. With this technique, CFs of different neuronal origins are differentially... 

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