Elastin-like proteins

Sandberg, L. B., Weissman, N., and Smith, D. W. The purification and partial characterization of a soluble elastin-like protein from copper-deficient porcine aorta. Biochemistry 8, 2940-2945 (1969). 

Girotti et al. (2004) have designed and bio-produced elastin-like protein polymers (ELP) which contain biofunctional motifs with cell adhesion sequences required for tissue-engineering applications. The protein polymer contained periodically spaced fibronectin CS5 domains enclosing the cell attachment sequence REDV. The overall sequence,... 

Elastic fibers are usually found in tissues rich in smooth muscle or tissues containing fibroblasts possessing some of characteristics of smooth muscle cells (4). There is a recent report, however, that suggests elastin-like proteins may be secreted from chondrocytes (25). When elastin is secreted, it is accompanied by other proteins that appear to be important to its alignment into fibrils. One of these proteins is referred to as microfibrillar protein (cf. Table I, ref. 2). When elastin is secreted, it combines with the microfibrillar protein to form a complex which is initially rich in the microfibrillar protein. 

Silk. Structural studies of the model peptides of Bombyx mori silk-elastin like protein were undertaken using solid state NMR. Detailed structural analyses were performed using deconvolution subroutines assuming Gaussian line shapes for the Ala peaks. 

Nagarsekar, A., Crissman, J., Crissman, M. et al. (2002) Genetic synthesis and characterization of pH-and temperature-sensitive silk-elastin-like protein block copolymers. Journal of Biomedical Materials Research, 62,195-203. 

Dandu, R., Von Cresce, A., Briber, R. et al. (2009) Silk-elastin-like protein polymer hydrogels Influence of monomer sequence on physicochemical properties. Polymer, 50, 366-374. 

Hatefi, A., Cappello, J. and Ghandehari, H. (2007) Adenoviral gene delivery to solid tumors by recombinant silk-elastin-like protein polymers. Pharmaceutical Research, 24,773-779. 

Cresce, A. V., Dandu, R., Burger, A. et al. (2008) Characterization and real-time imaging of gene expression of adenovirus embedded silk-elastin-like protein polymer hydrogels. Molecular Pharmaceutics, 5, 891-897. 

Ehnerman, A.A., CappeUo, J., Ghandehari, H. and Hoag, S.W. (2002) Solute diffusion in genetically engineered silk-elastin-like protein polymer hydrogels. Journal of Controlled Release, 82,277-287. 

Ner, Y., Stuart, J.A., Whited, G. and Sotzing, G.A. (2009) Electrospinning nanoribbons of a bioengineered silk-elastin-like protein (SELF) from water. Polymer, 50, 5828-5836. 

Qiu, W.G., Teng, W.B., CappeUo, J. and Wu, X. (2009) Wet-spinning of recombinant SUk-elastin-like protein polymer fibers with high tensile strength tmd high deformability. Biomacromolecules, 10, 602-608. 

Anumolu, R., Gustafson, J.A., Magda, J.J. et al. (2011) Fabrication of highly uniform nanoparticles from recombinant sUk-elastin-like protein polymers for therapeutic agent delivery. ACS Nano, 5, 5374-5382. 

Price, R., Gustafson, J., Greish, K. et al. (2012) Comparison of silk-elastin-like protein polymer hydrogel and poloxamer in matrix-mediated gene deUvery. International Journal of Pharmaceutics, 427, 97-104. 

Xia, X., Xu, Q., Hu, X. et al. (2011) Tunable self-assembly of genetically engineered sdk-elastin-like protein polymers. Biomacromolecules, 12,3844—3850. 

Collins, T., Azevedo-SUva, J., da Costa, A., Branca, F., Machado, R. and Casal, M. (2013) Batch production of a silk-elastin-like protein in E. coli BL21(DE3) key parameters for optimisation. Microbial Cell Factories, 12, 21. 

Figure 16 Three-dimensional cell culture constructs derived from elastin-mimetic polypeptides, (a) Photograph of a chemically cross-linked scaffold derived from an elastin-like, protein-engineered biomaterial.

Reactive noncanonical amino adds have also been integrated into elastin-like protein polymers to enhance their application as biomaterials. The Tinell laboratory has used the photoreactive amino add p-azidophenylalanine to pattern elastin-like protein polymers on surfaces. " Zhang et d. used p-azidophenylalanine to photochemically cross-link an elastin-leudne zipper protein to a glass surface. A target protein linked to a second leudne zipper domain can then be captured from solution and patterned on the surface by dimerization of the leucine zipper domains. Carrico et al. used similar p-azidophenylalanine-fiinctionalized elastin protein polymers... 

VPGIG)2VPGKG[Pg.3527]

Alternate strategies to control the mechanical properties of elastin-like proteins, via combination with amino acid sequences from different fibrous proteins, have also been explored. Combining the temperature-responsiveness... 

Protein engineering strategies have also allowed the incorporation of biological function into elastin-like protein polymers and SELPs. The incorporation of cell-binding amino acid seqnences, such as RGD (82,142) and REDV (from the CS5 domain of fibronectin) (66) (Table 2), permits endothelial cell adhesion to... 

Caves, J.M., Kumar, V.A., Martinez, A.W., Kim, J., Ripberger, C.M., Haller, C.A., Chaikof, E.L., 2010. The use of microfiher composites of elastin-like protein matrix reinforced with synthetic collagen in the design of vascular grafts. Biomaterials 31, 7175-7182. 

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