Effect on cell viability

De Nicola, M. et al. (2008) Carbon nanotubes on Jurkat cells effects on cell viability and plasma membrane potential. Journal of Physics Condensed Matter,... 

Treatment of cells with concentrations of vincristine or vindesine that produce relatively little effect on cell viability results in an accumulation of cells in the M and Gj (gap after DNA synthesis) phases of the cell cycle. The cellular effects, as measured by techniques such as flow... 

Nanoparticles formulated with PLGA have been shown to be rapidly uptaken by the endothelial cells, the uptake was shown to depend on the nanoparticle concentration and the particles where mainly shown to localize in the cytoplasm (207). These nanoparticles were also shown to be biocompatible with the cells with no effect on cell viability (207). This is important due to the fact that endothelium is an important target for gene therapy in a number of disorders including angiogenesis, atherosclerosis, tumor growth, myocardial infarction, limb and cardiac ischemia, restenosis (207). 

Reactive oxygen species production is largely catalyzed by transition metals (especially copper and iron), and oxidative stress plays a critical role in AD pathogenesis. In one study, the association of metal levels and Ap toxicity was demonstrated by (i) the effect on cell viability by metal alone and in the combination with APP and Ap, (ii) Ap-induced neurotoxicity relevant to oxidative stress indicated by ROS production, and (iii) APPsw cells expressed APP and generated Ap, so that Ap Cu2+ and APP Cu2+ can catalyze more ROS generation than APP cells that only expressed APP. 

As with all cell lines, it is important to maintain consistent culture conditions for the Hep AD38 cell line. In particular, it is important to validate one s lots of FBS and medium prior to use in the assay to ensure good and consistent virus production. In addition, the authors find that different lots of trypsin EDTA have variable effects on cell viability. Thus, before using a new lot of trypsin EDTA, its effect on cell viability should be determined. 

Metabolic Flux of Unnatural Sugar Analogs and Effects on Cell Viability... 

In the present authors laboratory, in-vitro tests have been carried out on numerous alloys and pure metals in order to determine their effect on cell viability and their capacity to induce inflammatory reactions (Table 5.4). 

The massive influx of Na and Ca result in strong cell membrane depolarizations. In many cases, cell depolarization produces the activation of voltage-gated Ca and Na channels (when MTX is tested in excitable cells), providing extra pathways for the further influx of these cations. Eventually, the cell deteriorates and dyes when its [Ca Ji buffering mechanisms are overwhelmed by the dramatic Ca uptake. In all reported studies, prolonged exposure to MTX has deleterious effects on cell viability. 

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