Effect of Growth and Culture Conditions on Ganglioside Metabolism

Effect of Growth and Culture Conditions on Ganglioside Metabolism 

Culture Conditions and Selection. The effects of other culture parameters have recently been reviewed (Mora, 1973). In general, variation of culture media, pH, and serum had little or no effect on ganglioside metabolism. Contact-inhibited cells grown under crowded conditions for a number of passages whereby the cells became less 

FIGURE 4. Effect of cell density on glycosyltransferase activities. (Reproduced with permission from Fishman et al., 1972.) 

Another aspect of culture conditions is clonal selection. Subclones of a NIL hamster cell line were reported to have extensive differences in glycolipid composition (Sakiyama et aL, 1972). However, another group 

Sialic-Add-Containing Glycolipids in Transformed Cells Obtained 

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