Ganglioside metabolism

The importance of gangliosides in human health is evident in neurodegenerative diseases associated with defects in the degradation of gangliosides. It is well known that serious neurological deficits develop in humans with aberrant GSLs or ganglioside metabolism. Most... 

Sango, K., Yamanaka, S., Hoffmann, A., Okuda, Y., Grinberg, A., Westphal, H., McDonald, M. P., Crawley, J. N., Sandhoff, K., Suzuki, K., and Proia, R. L., Mouse models of Tay-Sachs and Sandhoff diseases differ in neurologic phenotype and ganglioside metabolism, Nat Genet 11 (1995) 170-176. 

Desplats PA, Denny CA, Kass KE, Gilmartin T, Head SR, Sutcliffe JG, Seyfried TN, Thomas EA (2007) Glycohpid and ganglioside metabolism imbalances in Huntington s disease. Neurobiol Dis 27 265-277... 

MagUone V, Marchi P, Di Pardo A, et al. Impaired ganglioside metabolism in Himtington s disease and neuro-protective role of GMl. / Neurosci. 2010 30(ll) 4072-4080. 

Van Echten, G., and Sandhoff, K., 1993, Ganglioside metabolism Enzymology, topology and regulation, J. Biol. Chem. 268 5341-5344. 

Saito, M., and Yu, R. K., 1992, Role of myelin-associated neuraminidase in the ganglioside metabolism of rat brain myelin, J. Neurochem. 58 83-87. 

III. GANGLIOSIDE METABOLISM IN CULTURED MOUSE CELL LINES... 

C. Effect of Growth and Culture Conditions on Ganglioside Metabolism... 

Culture Conditions and Selection. The effects of other culture parameters have recently been reviewed (Mora, 1973). In general, variation of culture media, pH, and serum had little or no effect on ganglioside metabolism. Contact-inhibited cells grown under crowded conditions for a number of passages whereby the cells became less... 

If this altered ganglioside metabolism is related to some viral function, is it a function involved in transformation, productive infection, or both Mouse cells are permissive for polyoma virus and are rarely transformed by it (cf. Eckhart, 1969). Upon viral infection, the cells undergo a series of discrete events which result in cell lysis and release of new virions (cf. Weil and Kara, 1970). These include the appearance of viral-specific T-antigen, induction of cellular and viral DNA synthesis which is maximum at 25-30 hr postinfection, synthesis of viral coat proteins and new virions (present at 50 hr.), and, finally, cell lysis and virus release (5-7 days after infection). Swiss 3T3 and TAL/N cells were treated with sufficient polyoma virus to ensure infection of all cells. Hematoside UDP-GalN AcA-acetylgalactosaminyl-transferase was then determined at two key times after infection (Table V). The activity of this enzyme was similar to that seen in mock-infected cells. The results indicate that the reduced aminosugar transferase activity in virally transformed cells is specifically related to some transforming function of the viruses and is not a consequence of lytic infection of the cell. 

B. Ganglioside Metabolism in Flat Revertant Cell Lines... 

Since the altered ganglioside metabolism is found in virally transformed cell lines which have altered growth properties in culture and in... 

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