EDTA ethylene diamine tetraacetic

In this laboratory, we also include the metal ion chelators EDTA (ethylene diamine tetraacetic acid binds, e.g., Mg2 1 -ions) and EGTA (ethylene glycol-bis(2-aminoethyl)-Al,iV,iV/,iV/,-tetraacetic acid binds, e.g., Ca2+-ions) in our lysis buffers. These agents help prevent phosphatase action (by the metal ion-dependent phosphatase PP2C, which is not inhibited by microcystin-LR), metal (Ca2+) dependent proteinases, and protein kinases, which require divalent cations such as Mg2 1 (and, in some cases, also Ca2+). We also use a mix of proteinase inhibitors that inhibit a broad range of proteolytic enzymes, including serine and cysteine proteinases. 

APHA = American Public Health Association EDTA = ethylene diamine tetraacetic acid EPA = Environmental Protection Agency Kl = potassium iodide OSHA = Occupational Safety and Health Administration UVA/IS = ultravioletA/isible light... 

The treatment of complex ion equilibria in solution is analogous to the treatment of weak acids. One of the best known chelators is the well-known EDTA (ethylene diamine tetraacetic acid). The metal stability constants for EDTA are very high, which indicates strong complexes. Various other compounds are available with high metal-stability constants for agricultural or environmental uses. Some of the more important ones are DPTA (diethylene triamine pentaacetic acid), CyDTA (cyclohexane diamine tetraacetic acid), EDDA [ethylene diamine di (0-hydroxyphenyl acetic acid], or Chel-138. 

Thrombin EDTA (ethylene diamine tetraacetic acid)... 

Abbreviations MIANS, 2-(4 -maleimidylanilino)naphthalene-6-sulfonic acid PBS, phosphate buffered saline 20 mM sodium phosphate containing 150 mM sodium chloride, pH 7.2 EDTA, ethylene diamine tetraacetic acid DTT, dithiothreitol GuHCl, guanidine hydrochloride 2-ME, 2-mercaptoethanol RB2L-MIANS, ricin B-chain covalently blocked by two affinity ligands and MIANS labeled TEA, Trifluoroacetic Acid. 

The following abbreviations will be used ADH—alcohol dehydrogenase DPN — diphosphopyridine nucleotide DPNH—reduced diphosphopyridine nucleotide OP—1,10-phenanthroline 8-OHQ—8-hydroxyquinoline a,a -D—a,a -dipyridyl 8-OHQ5SA—8-hydroxyquinoline-5-sulfonie acid TU—thiourea EDTA—ethylene-diamine tetraacetic acid zincon —2-carboxy-2 -hydroxy-5-sulformazylbenzene diamox — sodium 2 - acetylamino -1,3,4- thiodiazole - 5 - sulf onamide NaDDC — so -dium-diethyldithiocarbamate CGP—carbobenzoxyglycyl-L-phenylalanine. 

Ac-MBOCA = N-acetyl-4,4 -methylenebis-(2-chloroaniline) ECD = electron capture detection ED = electrochemical detection EDTA = ethylene diamine tetraacetic acid El = electron impact FID = flame ionization detection GC = gas chromatography ... 

The cytotoxicity assay is usually performed by determining the viability of suitable cell lines in the presence of polymers. For this test, 3-5 mm discs of polymer film are cut and sterilised under standard conditions (at 121 C and 6.8 kg (15 lb) pressure for 15 min). The cell growth in the presence of the polymer films is measured under a controlled atmosphere (CO2 incubator, 37"C) using an appropriate culture medium, supplemented by 10% fetal bovine serum and penicillin-streptomycin antibiotic solution. Confluent monolayers are propagated by trypsinisation (0.25% trypsin and 0.02% EDTA, ethylene diamine tetraacetic acid) and re-plated at 2 x 10 cells/mL in a sterile polystyrene cell culture plate, then incubated for 24,48 and 72 h. The morphology of the cells is analysed by light naicroscopy (Leica) after... 

The following abbreviations for the ligands are used throughout the paper CyDTA = trans-cyclohexane diamine tetraacetate, EDTA = ethylene-diamine tetraacetate, 1.3-PDTA = 1.3-propanediaminetetraacetate, EDDaDp = ethylenediamine-N,N -dipropionate-N,N -diacetate, IDA = iminodiacetate, MIDA = N-methyl-iminodiacetate, EIDA = N-ethyl-iminodiacetate, n-PIDA = N-n-propyl-iminodiacetate, n-BIDA = N-n-butyl-iminodiacetate. 

Impregnating agents that are able to form complexes with the sample molecules to be separated. Examples include organic substances that are able to act as ligands in a complex-formation process, such as EDTA (ethylene diamine tetraacetic acid). These substances can be used to separate antibiotics (197,198) and metal ions (199,200). 

EDFA (erbium-doped fiber amplifier), 341 EDTA (ethylene diamine tetraacetic acid) for polymeric gels synthesis, 89 solution process, 62, 63, 64 EDTA-EG technique as a polymerizable method, 89 gelation process, 89 EDX for bimetallic catalysts, 520 EELS studies on barium titanate, 85 elastic modulus, 250 eleclrocatalytic films, 94 electrochromic coatings, 292 films, 82... 

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