E3 binding protein

Fig. 14.4. Expression of subunits of the pyruvate dehydrogenase complex during the development of A suum. Homogenates of different A suum larval stages and adult tissues were immunoblotted with polyclonal antisera prepared against individual subunits of the A suum PDC isolated from adult muscle, as described in detail in Klingbeil etal. (1996). UE, unembryonated egg M, adult body wall muscle p45, E3-binding protein (E3BP).

Eipi (Wheelock etal., 1991) Eipil (Huang and Komuniecki, 1997) E3BP, E3-binding protein (p45) E3 ER, enoyl CoA reductase (Duran etal. 1993, 1998) AAT, adenine nucleotide translocase a-tubulin. UE, unembryonated egg L1, first-stage larva L2, second-stage larva L3, third-stage larva M, adult muscle ... 

E (entgegen) configuration 43 E3 binding protein 796 Eadie-Hofctee plot 460 E-cadhedrin 574 Echinodermata 25 Ectoderm 23 Ectoenzymes 409 Ectopic proteins 573 Edelman, Gerald M. 84 Edman degradation 118 EDTA. See Ethylenediaminetetraacetic acid Effector(s) of allosteric enzymes 473-475 EF-hand motif 313, 317 EGF (epithelial growth factor), definition of 577... 

Hiromasa, Y., Fujisawa, T., Aso. Y, and Roche, T. E. 2004. Organization of the cores of the maminalian pyruvate dehydrogenase complex formed by E2 and E2 plus the E3-binding proteins and their capacities to bind the El and E3 components. J. Biol Chem. 279 6921-6933. 

In the mammalian enzymes the interactions are even more complicated since while the El component interacts with the E2 component, the E2 component does not directly interact with the E3 component, rather the information is relayed from E2 to the E3-binding protein (E3BP), which in turn interacts with the E3 component. 

Coen, P. G., McDonald, E. R., 3rd, Herman, J. G., and El-Deiey, W. S. Tat-binding protein-1, a component of the 26 S proteasome, contributes to the E3 ubiquitin ligase function of the von Hippel-Lindau protein. Nat Genet... 

The conical objects protruding in are the E3 molecules. The arrow points to a binding site for attachment of the binding protein to the core. Courtesy of J. K. Stoops et al.299... 

An indirect measure of the concentration of a specific binding protein can be obtained by adding increasing amounts of thyroxine to serum which has been partitioned by electrophoretic means. Saturation, or the maximal binding capacity is a function of the number and capacity of binding sites available [1/mole (013) in the case of TBPA]. The concentration of binding sites is proportional to the amount of thyroxine necessary to saturate the protein. Such methods have been described (010). Reverse-flow techniques can be used to eliminate trailing albumin from the TBG (E3). 

Burgert H-G, Kvist S (1987) The E3/19K protein of adenovirus type 2 binds to the domains of histocompatibility antigens required for CTL recognition. EMBO J 6 2019-2026 Burgert H-G, Maryanski JL, Kvist S (1987) E3/I9K" protein of adenovirus type 2 inhibits lysis of cytolytic T lymphocytes by blocking cell-surface expression of histocompatibility class I antigens. Proc Natl Acad Sci USA 84 1356-1360... 

Flomenberg P, Gutierrez E, Hogan KT (1994) Identification of class I MHC regions which bind to the adenovirus E3-19k protein. Mol Immunol 31 1277-1284 Flomenberg P, Piaskowski V, Truitt RL, Casper JT (1996) Human adenovirus-specific CD8+ T-cell responses are not inhibited by E3-19K in the presence of gamma interferon. J Virol 70 6314-6322 Fox JP, Hall CE, Cooney MK (1977) The Seattle virus watch. VII Observations of adenovirus infections. Am J Epidemiol 105 362-386... 

Sester M, Burgert H-G (1994) Conserved cysteine residues within the E3/19K protein of adenovirus type 2 are essential for binding to major histocompatibility complex antigens. J Virol 68 5423-5432 Sester M, Feuerbach D, Frank R, Preckel T, Gutermann A, Burgert H-G (2000) The amyloid precursorlike protein 2 associates with the major histocompatibility complex class i molecule K. J Biol Chem... 

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