Dynamic imaging, micro

Abstract. Electroanalytical methods are highly compatible with micro- and nano-machining technology and have the potential of invasive but non-destmctive cell analysis. In combination with optical probes and imaging techniques, electroanalytical methods show great potential for the development of multi-analyte detection systems to monitor in real-time cellular dynamics. 

Roberts, H.C., et al.. Quantitative measurement of micro-vascular permeability in human brain tumors achieved using dynamic contrast-enhanced MR imaging correlation with histologic grade. AJNR Am J Neuroradiol, 2000. 21(5) p. 891-9. 

Three types of instrumentation exist for dynamic SIMS non-imaging ion probes, direct-imaging ion microanalysers and scanning ion microprobes-micro-scopes. Non-imaging ion probes are often an accessory of Auger electron spectroscopy (AES), electron spectroscopy for chemical applications (ESCA), or electron microscopy systems and allow a point analysis. Imaging equipment allows a point-to-point analysis of the surface with a primary beam of size 10—300 pm (microanalysers) or below 10 pm (microprobes-microscopes). 

Environmental-cell in situ specimen holders provide the possibility of investigating a nanoparticle s dynamic behaviour in real time, within a liquid or gaseous environment. These holders employ Micro-Electro-Mechanical Systems (MEMS) chips containing thin impermeable silicon nitride windows to isolate the liquid or gas from the microscope while remaining electron transparent. STEM is widely employed in environmental-cell studies as it allows good contrast when imaging through thick liquid layers.For example, it has been shown to be... 

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