Transfer DNA

Eor example, the technique of Southern blotting was developed (68) for use with agarose gel electrophoresis of DNA fragments. Southern blots are designed to detect specific sequences of DNA. After electrophoresis is complete, the DNA is denatured and the single stranded DNA transferred to the specially prepared nitrocellulose paper. The nitrocellulose is then incubated with radioactive RNA or DNA complementary to those DNA sequences of interest. After the nitrocellulose has been sufftciendy incubated with the radioactive complementary DNA, autoradiography is used to identify the fragments of interest. 

Mbisa JL, Barr R, Thomas JA, Vandegraaff N, Dorweiler IJ, Svarovskaia ES, Brown WL, Mansky LM, GoreUck RJ, Harris RS, Engelman A, Pathak VK (2007) Human immunodeficiency virus type 1 cDNAs produced in the presence of APOBEC3G exhibit defects in plus-strand DNA transfer and integration. J Virol 81(13) 7099-7110... 

Significant strides have been made in past decades with development of new therapies that have led to the ever-lengthening CF life-span. Since the discovery of the CF gene and the CFTR protein defect, research has focused on gene therapy as a way to restore normal CFTR function through DNA transfer. 

Sohotra Sarkar The only part I was thinking of was a minor extension, to get a theory of transmission that was beyond just DNA transfer. 

Sonawane ND, Szoka FC Jr, Verkman AS (2003) Chloride accumulation and swelling in endo-somes enhances DNA transfer by polyamine-DNA polyplexes. J Biol Chem 278 44826-44831... 

Byk G, Dubertret C, Escriou V, et al. Synthesis, activity, and structure-activity relationship studies of novel cationic lipids for DNA transfer. J Med Chem 1998 41(2) 229-235. 

Substantial work has been undertaken on the potential of the CPP peptides TAT and Antp/penetratin for gene delivery. Some workers have found that TAT alone has the ability to promote DNA transfer (80,87) but their combination with liposomes appears to produce a more robust vector system. For example, a 15- to 25-fold increase in uptake was reported in liposomes modified with either TAT or the drosophila CPP, Antp, in... 

Agrobacterium-mQdi iQd DNA transfer technique and direct gene transfer into protoplasts on the basis of the electroporation technique have frequently been used for the gene transfer methods in plants. However, these methods were hardly adapted to monocotyledonous plants or to varieties in which regeneration system was not established. These deficiencies found in gene transfer techniques will be largely overcome if pollen could be used as a DNA vector. 

VirB Part of secretory system required for T-DNA transfer... 

T-DNA transfer to corn, a plant species that is difficult to transform by... 

DNA Transfer Mediated by T-DNA oi Agrobacterium tumefaciens and Other Approaches... 

Schiedner, G. (1998). Genomic DNA transfer with a high-capacity adenovirus vector results in improved in vivo gene expression and decreased toxicity. Nat. Genet., 18, 180-183. 

Cannio, R. Contursi, P. Rossi, M. Bartolucci, S. Thermoadaptation of a mesophilic hygromycin B phosphotransferase by directed evolution in hy-perthermophilic Archaea selection of a stable genetic marker for DNA transfer into Sulfolobus solfataricus. Extremophiles, 5, 153-159 (2001)... 

One result of DNA transfer from Hfr into F bacteria is sometimes the introduction of a complete copy of the F agent into the female bacterium. Since this con-... 

The specific properties of any protein are due to the specific sequence of amino acids in its polypeptide chains. This sequence is determined by the genetic information carried by the sequence of DNA nucleotides. DNA transfers the information to messenger RNA, which serves as the template for protein synthesis. 

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