DNA-dependent protein kinase catalytic subunit

Hartley, K. O., Gell, D., Smith, G. C., et al., DNA-dependent protein kinase catalytic subunit A relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. Cell 82, 849-856 (1995). 

Oka A, Takashima S, Abe M, Araki R, Takeshita K. 2000. Expression of DNA-dependent protein kinase catalytic subunit and Ku80 in developing human brains Implications of DNA-repair in neurogenesis. Neurosci Lett 292 167-170. Okabe S. 2007. Molecular anatomy of the postsynaptic density. Mol Cell Neurosci 34 503-518. 

DNA-dependent protein kinase catalytic subunit, was obtained by cDNA cloning from a monoclonal antibody 18-2-ptoducing cell line 24). ScFvs were purified by conventional or anti-E tag affinity chromatognq>hy for use in enzyme-linked immunosorbent assay (ELISA), immunofluorescence assays, and microinjection experiments. 

Gene expression in benzene-exposed workers by microarray analysis of peripheral mononuclear blood cells induction and silencing of CY-P4F3A and regulation of DNA-dependent protein kinase catalytic subunit in DNA double strand break repair. Chem Biol Interact 184 207-211... 

Exposure of cells to nitric oxide resulted in a 4 -5-fold increase in expression of the DNA-dependent protein-kinase catalytic subunit (Xu et al. 2000), one of the key enzymes involved in repairing double-stranded DNA breaks. This NO-mediated increase in enzymatically active DNA-PK not only protected cells from the toxic effects of NO, but also provided crossprotection against clinically important DNA-damaging agents, such as X-ray radiation, doxorubicin, bleomycin and cisplatin. 

Ataxia telangiectasia is an autosomal recessive disease characterized by neurologic, endocrine, and hepatic abnormalities, as well as a predisposition to malignancy (119). The defect has been traced to a gene on chromosome 11, the ATM gene that codes for a phosphatidylinositol 3-kinase-like protein which is related to the catalytic subunit of DNA-dependent protein kinase. This protein has a role in signal transduction, DNA repair, and control of the cell cycle (120). Affected patients have a defect in cell-mediated immunity. A decrease in semm IgA is seen in a majority of affected patients. IgG2 or total IgG and IgE levels may be decreased, with an increase in IgM. Patients are susceptible to chronic respiratory... 

Peterson, S., Kurimasa, A., Oshimura, M., Dynan, W., Bradbury, E., and Chen, D. (1995). Loss of the catalytic subunit of the DNA-dependent protein kinase in DNA double-strand-break-repalr mutant mammalian cells. Proc. Natl. Acad. Sci. USA 92, 3171-3174. 

Within seconds after induction, the DSB is bound by a heterodimer of Ku70 and Ku80, which keeps the ends in proximity and immediately recruits a third large protein, the catalytic subunit (DNA-PKcs), into a holocomplex called DNA-dependent protein kinase (DNA-PK). DNA-PK executes several key functions and orchestrates the entire process, which is, hence, also called DNA-PK-dependent nonhomologous endjoin-... 

In prokaryotic cells, cAMP binds to catabolite regulatory protein (CAP), which then binds to DNA and affects gene expression (Chapter 26). In eukaryotic cells, cAMP binds to cAMP-dependent protein kinase, which contains two regulatory (R) and two catalytic (C) subunits. Upon binding of cAMP, the catalytic subunits separate, become active. 

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