Distearoyl phosphatidylcholine DSPC

FIG. 10 Vibrational sum frequency spectrum of saturated monolayers of dilauroyl- (DLPQ, dimyristoyl- (DMPC), dipalmitoyl- (DPPC), and distearoyl-phosphatidylcholine (DSPC) at the D2O-CCI4 interface at ambient temperature in the region of the methylene and methyl symmetrical stretches. (From Ref 139, copyright American Chemical Society.)... 

LEH is primarily composed of a combination of saturated high-carbon phospholipids and cholesterol. Synthetic phospholipids replaced hydrogenated soy lecithin when the latter was found to induce several untoward biological responses (40). Current choice of a saturated high-carbon phospholipid is mostly between distearoyl phosphatidylcholine (DSPC, 55°C) and... 

Because the dynamics of phospholipid membranes have been well characterized using AF probes [8-13], fluorescence results obtained with hydrated human SC were compared to aqueous suspensions of unilamellar distearoyl-phosphatidylcholine (DSPC) vesicles. DSPC was also used because its phase transition temperature (55°C) is close to that of SC lipids (65 C). The microenvironment inside DSPC and SC membranes was studied by measuring fluorescence lifetimes, and shifts in emission maxima were compared to excitation maxima (Stokes shifts), along with quenching of a series of AF probes by iodide. Stokes shifts (Av) [6] were calculated as ... 

Figure 19.1. The general structures of some phospholipids, distearoyl-phosphatidylcholine (DSPC), 1, and distearoylphosphatidylethanolamine (DSPE), 2.

Egg phosphatidylcholine (PC), distearoyl phosphatidylcholine (DSPC), phospha-tidic acid (PA), and phosphatidyl glycerol (PG) (more than 99% pure) were from Lipid Products Nutfield. All other phospholipids (more than 98% pure), cholesterol, triolein (TO), stearylamine (SA), fluorescein isothiocyanate (FITC), and oil red O were from Sigma Chemical Company. Sepharose CL-4B and polyethylene glycol 6000 were obtained from Pharmacia. All other reagents were of analytical grade. 

DSPC distearoyl phosphatidylcholine DPPG dipalmitoyl phosphatidylglycerol MI mechanical index... 

Nonideal miscibility - in ideal mixtures, the components do not interact with each other, thus the properties of the mixture depend linearly on the corresponding property of each component, and on the composition of the mixture. However, in practice, two-component lipid mixtures behave nonideally, and this gives rise to anomalies. For example the phosphatidylcholines dimyristoyl PC (DMPC, C14 saturated fatty acids), dipalmitoyl PC (DPPC, C16 saturated fatty acids) and distearoyl PC (DSPC, Cl 8 saturated fatty acids) have their main... 

Abbreviations DMPC, DPPC, DSPC, dimyristoyl-, dipalmitoyl-,distearoyl-phosphatidylcholine DPPA, dipalmitoylphosphatidic acid PE, phosphatidylethanolamine PS, phosphatidylserine (U) and (M), unilamellar and multilamellar liposomes temperature of crystal liquid-crystal transition Diff. spectr., difference spectroscopy. 

DSPC, l,2-distearoyl-sn-3-glycero-3-phosphatidylcholine numbers preceding PC indicate wether both or only the second chain is substituted... 

The formation of liposomes [or better arsonoliposomes (ARSL)], composed solely of arsonolipids (Ars with R=lauric acid (C12) myristic acid (C14) palmitic acid (C16) and stearic acid (C18) (Fig. 1) have been used for ARSL construction), mixed or not with cholesterol (Choi) (plain ARSL), or composed of mixtures of Ars and phospholipids (as phosphatidylcholine [PC] or l,2-distearoyl- -glyceroyl-PC [DSPC]) and containing or not Choi (mixed ARSL), was not an easy task. Several liposome preparation techniques (thin-film hydration, sonication, reversed phase evaporation, etc.) were initially tested, but were not successful to form vesicles. Thereby a modification of the so called one step or bubble technique (8), in which the lipids (in powder form) are mixed at high temperature with the aqueous medium, for an extended period of time, was developed. This technique was successfiil for the preparation of arsonoliposomes (plain and mixed) (9). If followed by probe sonication, smaller vesicles (compared to those formed without any sonication [non-sonicated]) could be formed [sonicated ARSL] (9). Additionally, sonicated PEGylated ARSL (ARSL that contain polyethyleneglycol [PEG]-conjugated phospholipids in their lipid bilayers) were prepared by the same modified one-step technique followed by sonication (10). 

PLGA (D,L)-poly(lactic glycolic acid), PEG poly(ethylene glycol), DSPC distearoyl-L-a-phosphatidylcholine, DSPG distearoyl-L-a-phosphatidylglycerol) Aerodynamic diameter, Liposome vesicle size... 

DSP. See Sodium phosphate dibasic anhydrous DSP-2. See Sodium phosphate dibasic dihydrate DSP-7. See Sodium phosphate dibasic heptahydrate DSP-12. See Sodium phosphate dibasic dodecahydrate DSP-0. See Sodium phosphate dibasic anhydrous DSPC. See 1,2-Distearoyl-sn-glycero(3) phosphatidylcholine DSPP. See Sodium acid pyrophosphate DSS. See Dioctyi sodium suifosuccinate... 

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