Displays Fluorescent activated

The ability to display proteins on the surface of an organism has been exploited as a screening method, typically in conjunction with fluorescence-activated cell sorting (FACS) [37,47,48]... 

Becker, S., Schmoldt, H.U., Adams, T.M. et al. (2004) Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts. Current Opinion in Biotechnology, 15, 323-329. 

As indicated above, one major advantage of bacterial and other cell-based surface display formats lies in the ability to use fluorescence-activated cell sorting for high-throughput library screening. With modem FACS equipment, such as the Cytomation MoFlo or the FACS Vantage from Beckton-Dickinson, sorting rates of up to 100 000 events per second are possible [10]. 

The pentacyclic thioxanthylium salt 624 absorbs at 582 nm (emax 105 000 M-1 cm-1) with a fluorescence emission maximum at 600 nm. The dye displays photodynamic activity in vitro against chemosensitive hamster ovary cell lines (Equation 207) <2006BMC8635>. 

When the same flavonoids were tested on the mouse lymphoma cells, rotenone, chrysin, phloretin and epigallocatechin displayed a dose-dependent increase in the fluorescence activity ratio (FAR). These flavonoids caused... 

Backstrom, B.T., Brockelbank, J.A., and Rehm, B.H.A. (2007) Recombinant Escherichia coli produces tailor-made biopolyester granules for applications in fluorescence activated cell sorting functional display of the mouse interleukin 2 and myelin oligodendrocyte glycoprotein. BMC Biotech., 7, 3, Article Number 3. 

OPTICAL CHARACTERISTICS OF LIQUID CRYSTAL DISPLAYS THE FLUORESCENCE-ACTIVATED DISPLAY FLAD... 

---