Discovery toxicology application

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Drug discovery applications also provide insights for the eventual determination of the compounds in a forensic or toxicological setting this is another area in which multidimensional chromatography allows the direct injection of bodily fluids. For... 

Thin-layer chromatography (TLC) is one of the most popular and widely used separation techniques because it is easy to use and offers adequate sensitivity and speed of separations. Furthermore, multiple samples can be run simultaneously. It can be used for separation, isolation, identification, and quantification of components in a sample. The equipment used for performance of TLC, including applications of TLC in drug discovery process, is covered in Chapter 13. This technique has been successfully used in biochemical, environmental, food, pharmacological, and toxicological analyses. 

The are several clearance and toxicological aspects that have to be considered in the drug discovery process such as metabolic stability, enzyme selectivity, CYP inhibition and type of inhibition. Among these factors, the prediction of the site of metabolism has become one of the most successful parameters for prediction. The knowledge of the site of metabolism enhances the opportunity to chemically modify the molecule to improve the metabolic stability. There are several approaches based on database mining, chemical reactivity, protein interaction or both that have been developed for the prediction of this property, with different degree of success and applicability. 

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