Disaccharides chromatography

Defaye, K. and Garcia Fernandez, J.M., Protonic and thermal activation of sucrose an the oligosaccaride composition of caramel. Carbohydrate Res., 256, Cl, 1994. Ratsimba, V. et al.. Qualitative and qnantitative evaluation of mono- and disaccharides in D-fructose, D-glucose and sucrose caramels by gas-liquid chromatography-mass spectrometry di-D-fructose dianhydrides as tracers of caramel authenticity, J. Chro-matogr. A, 844, 283, 1999. 

Bugner, E. and Feinberg, M. (1992). Determination of mono and disaccharides in foods by interlaboratory study Quantitation of bias components for liquid chromatography. /. Assoc. Ojfic. Anal. Chem. 75,443-464. 

Acid hydrolysis of the basic hexasaccharide yielded the disaccharide 31. Its mobility in paper chromatography lay between those of the corresponding (1 — 3)-linked (from S10A) and /3-(l — 6)-linked (from S29) isomers, which is why it was assumed to be (1 — 4)-linked. The (1 — 2)-linked isomer was excluded, as the D-galactose residue that is part of 31 carries a D-galactopyranosyl group linked to 0-2 in the original polysaccharide, as will be discussed. 

Purification by flash chromatography yielded the pseudo-disaccharide as a glass... 

Module 3, Column and Mobile Phase Design (CMP). This is the core module for ECAT. It can currently specify i) analytical column and mobile phase constituents for reverse phase chromatography of common classes of organic molecules ii) reverse phase, ion exchange phase and hydrophobic interaction chromatography of proteins and peptides iii) a limited set of specialty classes of molecules best treated by straight phase chromatography (e.g., mono- and disaccharides). The rules for selection of the HPLC detector are under development within Module 3. Some of the rules for detector mobile phase compatibility are already encoded. A set of rules for detector selection is ready but not yet encoded. 

On the other hand, when intermediate 266 reacted with the ketone 270 (precursor of epoxide 263), a methylene-bridged disaccharide 271 was the only reaction product isolated in low yield (15%), due to difficulties in its purification by column chromatography. 

The ease of desilylation may permit the effluent to be collected and then hydrolyzed, and the product (a) transformed into a crystalline derivative, for example, a lactone into a phenylhydrazide,205 or (b) examined by thin-layer chromatography.206 In the case of a glycoside or disaccharide, regeneration of the parent compound permits the configuration of the glycosidic linkage to be determined by enzy-molysis.207... 

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