Digoxin antibodies

Fig. 7. Schematic diagram of digoxin antibody sensing electrode (a) PVC membrane containing digoxin-carrier conjugate (b) inner filling solution, 0.01 M KCI (c) plasticizer, dibutyl sebacate (d) digoxin antibodies. (From 152, with permission)...

M2. Mudgett-Hunter, M., Anderson, W., Haber, E., and Margolies, M. N., Binding and structural diversity among high-affinity monoclonal anti-digoxin antibodies. Mol. Immunol. 22, 477-488 (1985). 

Digoxin antibodies Digoxin and related cardiac glycosides One vial binds 0.5 mg digoxin indications include serious arrhythmias, hyperkalemia. 

K4. Keating, M. Y., and Rechnitz, C. A., Potentiometric digoxin antibody measurements with antigen-ionophore based membrane electrodes. Anal. Chem. 56, 801-806 (1984). 

Intestinal ischemia responds to verapamil and to anti-digoxin antibody (115). 

Erdmann E, Mair W, Knedel M, Schaumann W. Digitalis intoxication and treatment with digoxin antibody frag- 203. ments in renal failure. KUn Wochenschr 1989 67(1) 16-19. 

A monoclonal anti-digoxin antibody (mouse) used for the ELISA was produced and prepared by Sawada et al. [121]. An ELISA plate (96 well) was coated with ca. 100 ng/well digoxin-conjugated ovalbumin in 50 mM sodium bicarbonate buffer (pH 9.6) at 4 °C overnight. After washing with 10 mM phosphate-buffered saline (pH 7.2) (PBS) containing 0.5 ml/1 Tween 20 (T-PBS), the wells were blocked by PBS supplemented with 1 g/1 casein (C-PBS). Fifty pi samples serially... 

Table 17. Cross-reactivity of anti-digoxin antibody in ELISA...

This biosensor employs a potassium (K+) ion-selective electrode as the transducer, and chemical recognition involves the unh-digoxin antibody and a digoxin-crown ether conjugate. A diagram of the sensor is shown in Figure 7.5. 

In this device, a poly(vinyl chloride) (PVC) membrane separates an internal K+ solution from an external layer of digoxin antibody that is trapped at the sensor surface with a dialysis membrane. The digoxin-crown ether conjugate, which selectively binds K+, is present only in the PVC layer due to its hydrophobic properties. Competition between free digoxin (analyte) that freely crosses the... 

Fig. 124. Direct potentiometric digoxin antibody-sensing electrode. (Redrawn from Keating and Rechnitz, 1984).

R Stenzel, B Reckmann. Cross-reactivity of anti-digoxin antibodies with digitoxin depends on tracer structure. Clin Chem 38 2228, 1992. 

The drug of choice in severe, massive overdose with any cardiac glycoside is digoxin antibody, Digibind. These antibodies are sufficiently nonselective to bind a variety of cardiac glycosides. The other drugs are used in moderate overdosage associated with increased automatic-ity. The answer is (A). 

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