Digital imaging spectroscopy

A peculiar case is certainly that of the green fluorescent protein (GFP). Functional GFP variants have been identified by visual inspection of colonies based on the intrinsic fluorescence of GFP under UV illumination [24, 32 - 34]. To increase accuracy of signal quantification as well as throughput, digital image spectroscopy has been applied in some cases [32],... 

Digital Imaging Spectroscopy for Massively Parallel Screening of Mutants... 

DIS is a context-dependent acronym for either digital imaging spectrophotometer or digital imaging spectroscopy. In earlier publications, DIS is synonymous with FAS, an acronym for filter array spectrophotomer. 

Digital Imaging Spectroscopy on Pigment-Protein Complexes... 

Goldman, E. R. Youvan, D. C. (1992) An algorithmically optimized combinatorial library screened by digital imaging spectroscopy. Bio/Tcc/mo/ogy 10, 1557-1561. 

B. M. Altura and B. T. Altura, Role of magnesium and calcium in alcohol-induced hypertension and strokes as probed by in-vivo television microscopy, digital image microscopy, optical spectroscopy, P-NMR spectroscopy and a unique magnesium ion-selective electrode. Alcohol. Clin. Exp. Res., 1994,18,1057-1068. 

Beside the identification of single bacterial cell or spores by means of Raman spectroscopy, the localization of these cells inside partly complex matrices has to be performed. One approach is the combination of Raman spectroscopy, fluorescence spectroscopy and digital imaging techniques. This method was applied to detect traces of endospores and other biothreat organisms even in the presence of complex environmental matrices like bioaeroso-lic background, nasal mucin [67], or tap water [68], Another fully automated device was built to analyze bioaerosols in clean room environments, where prior to the Raman identification method a particle preselection took place [69]. 

Radio waves have the lowest energy (lowest frequency). We use them for radio and television communication, digital imaging, remote controls, and wireless linkages for laptop computers. Radio waves are also used in NMR spectroscopy and in magnetic resonance imaging (MRI) (Chapter 14). 

Analytical electron microscopy permits structural and chemical analyses of catalyst areas nearly 1000 times smaller than those studied by conventional bulk analysis techniques. Quantitative x-ray analyses of bismuth molybdates are shown from lOnm diameter regions to better than 5% relative accuracy for the elements 61 and Mo. Digital x-ray images show qualitative 2-dimensional distributions of elements with a lateral spatial resolution of lOnm in supported Pd catalysts and ZSM-5 zeolites. Fine structure in CuLj 2 edges from electron energy loss spectroscopy indicate d>ether the copper is in the form of Cu metal or Cu oxide. These techniques should prove to be of great utility for the analysis of active phases, promoters, and poisons. 

---