Design limitations surface preparation processes

Table 4 Summary of design limitations for selected surface-preparation processes ...

Table 4 Summarizes design limitations for surface preparation/cleaning processes...

Table 4 provides a summary of the design limitations of some surface-preparation and cleaning processes and indicates which design features to avoid. 

Consideration of the structure and distribution of cannabinoid receptors is critical to the successful application of immunocytochemical approaches to the identification of cannabinoid receptors in cells and tissues. CBi and CB2 receptors exhibit greatest type specificity at their extracellular amine-terminal domains. Thus, experiments designed for identification of cannabinoid type-specific receptor expression at the protein level should entail use of affinity-purified antibodies directed against the amine-terminal domains. Use of such antibodies is also critical for the identification of cell surface expression of cannabinoid receptors. On the other hand, if assessment of expression of fully processed receptor protein is required, then antibodies directed against carboxy-terminal domains of the CBi or CB2 should be considered. Immunocytochemical methods in which such antibody preparations are considered should include a limited cell/tissue solubilization so as to allow for adequate antibody penetration. A list of commercially available antibodies to domains of CBi and CB2 receptors is included in Table 1. In addition, various laboratories have applied these commercially available antibodies (or have developed their own antibodies) to the identification of cannabinoid receptors at the fluorescence, light, and electron microscopy levels. Table 2 lists references in which immunocytochemical approaches have been utilized for the identification of cannabinoid receptors in a variety of tissues and cell types. 

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