Deoxyribonucleic acid polymerase chain reaction

FIGURE 6 The PCR process. Courtesy of PE Cetus Instruments. (From Schantield, M. S. (2000). Deoxyribonucleic Acid/Polymerase Chain Reaction. In Encyclopedia of Forensic Sciences (Siegel, J. A., Saukko, P. J., and Knupfer, G. C., eds ), Academic Press, London, p. 517.)... 

List of Abbreviations PCR, polymerase chain reaction RT-PCR, reverse transcription polymerase chain reaction DNA, deoxyribonucleic acid RNA, ribonucleic acid RNase, ribonuclease mRNA, messenger RNA GABAa, y-aminobutyric acid type A cRNA, copy RNA dNTPs, deoxy nucleoside triphosphates MMLV, Mouse Moloney murine leukemia vims RT, reverse transcriptase bp, base pair Tm, melting temperature DEPC, diethylpyrocarbonate OD, optical density mL, milliliter SA-PMPs, streptavidin paramagnetic particles dT, deoxy thymidine DTT, dithiothreitol DNase, deoxyribonuclease RNasin, ribonuclease inhibitor UV, ultraviolet TBE, Tris-borate, 1 mM EDTA EDTA, ethylenediaminetetraacetic acid Buffer RET, guanidium thiocyanate lysis buffer PBS, phosphate buffered saline NT2, Ntera 2 neural progenitor cells... 

Immunocapture-polymerase chain reaction (IC-PCR) is a synthesis of two commonly used diagnostic tools. This method exploits the high-affinity binding of antibodies to provide a facile method of purification, usually from a complex matrix, supplying the substrate for PCR detection. PCR exponentially amplifies a deoxyribonucleic acid (DNA) template in a temperature-dependent fashion by the annealing of oligonucleotide primers, enzymatic extension of bound primers by a heat-stable polymerase, followed by denaturation of... 

Researcher supervising an automated probe that is distributing DNA (deoxyribonucleic acid) samples among an array of microplates. This is part of an automated polymerase chain reaction (PCR) process, carried out to amplify the amount of DNA present in the samples. (Pascal Goet-gheluck/Photo Researchers, Inc.)... 

Alexander, J., Tumor suppressor loss in pituitary tumors. Brain Pathol., 11, 342-355 (2001). Evans, C.O., Young, A.N., Brown, M.R., Brat, D.J., Parks, J.S., Neish, A.S. and Oyesiku, N.M., Novel patterns of gene expression in pituitary adenomas identified by complementary deoxyribonucleic acid microarrays and quantitative reverse transcription-polymerase chain reaction. J. Clin. Endocrinol. Metab., 86, 3097-3107 (2001). 

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