Deoxyribonucleic acid extraction

Romig, W. R. Infectivity of Bacillus subtilis bacteriophage deoxyribonucleic acids extracted from mature particles and from lysogenic hosts. Bact. Rev. 32, 349-357 (1968). 

Deoxyribonucleic acid (from plasmids). Purified by two buoyant density ultracentrifugations using ethidium bromide-CsCl. The ethidium bromide was extracted with Et20 and the DNA was dialysed against buffered EDTA and lyophilised. [Marmur and Doty J Mol Biol 5 109 1962 Guerry et al. J Bacteriol II6 1064 1973.] See p. 504. 

In this connection, it must also be borne in mind that the deoxyribonucleic acids subjected to analysis have probably not been homogeneous. Deoxyribonucleic acids have been fractionated by making use of their different solubilities in normal saline,186 by extracting thymus nucleo-his-tone with sodium chloride solutions of increasing concentration,187 by ion-exchange,187 and also by adsorption of the polynucleotide onto histone immobilized on a kieselguhr support.123 It is possible, however, that these are artefacts, since it has been shown that deoxyribonucleic acid fractions extracted from calf-thymus nucleohistone may or may not vary in composition according to the previous treatment of the material.188... 

Our studies have shown that cotton dust extracts contain deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) (27). DNA is present in varying concentrations in cardroom cotton dust (28) and emanates from bacteria, fungi, protozoa and plant cells. DNA might be important in the pathogenesis of byssinosis, as minute amounts can cause complement conversion. 

Information concerning the method of manufacture may be presented in the form of a flow chart. Supply a brief description of the methods of isolation (e.g., synthetic process, fermentation, extraction, recombinant deoxyribonucleic acid [DNA] procedure) and purification (solvent recrystallization, column chromatography, distillation). Include all synthetic pathways that have been adequately characterized during the investigational stages of drug development. 

Kornberg s work on the biosynthesis of deoxyribonucleic acid has shown that enzymes in Escherichia coli extracts catalyze the formation of the 5-triphosphates of 2-deoxyadenosine, 2-deoxyguanosine, 2-deoxycyti-dine, and thymidine from the corresponding monophosphates in the presence of adenosine 5-triphosphate, but fail to catalyze phosphorylation of deoxyuridine 5-phosphate this finding could explain why uracil is not a constituent of deoxyribonucleic acid. 

After extraction from the extracellular compartment, the bulk of the substrates is used for the production of energy. During rest in the fed state, the substrates taken up will be stored in intracellular depots from which they are released when the supply of exogenous substrates is limited. Part of the substrates absorbed will be utilized for the synthesis of macromolecules such as proteins and deoxyribonucleic acids (DNA), or the production of compounds crucial for cellular membranes, like phospholipids and cholesterol. The latter are typical examples of anabolic, energy consuming processes. 

Ono, K. Nakane, H. Meng, Z.H. Ose, Y. Sakai, Y. Mizuno, M. Differential Inhibitory Effects of Various Herb Extracts on The Activities of Reverse Transcriptase and Various Deoxyribonucleic Acid (DNA) Polymerase. Chem. Pharm. Bull. 1989, 37, 1810-1812. 

Genomic deoxyribonucleic acid (DNA) was extracted from cell lines with the DNeasy tissue kit (Qiagen, Valencia, CA). 

Deoxyribonucleic acid (DNA) from any source can be used in Pyrosequencing assays (see Note 1). Commonly used kits for DNA extraction, including Centra and Qiagen, do not inhibit the assay. 

Medical applications of alkaloids led to the production of drugs and drug components. They can be based on a pure natural alkaloid, as in the ease of extracts. Purified alkaloids, partially and even totally synthesized compounds based on the natural alkaloid structure, are also used. Chemically modified alkaloids are yet another example, and their new structure effects stronger biological activity. Moreover, the trend in the science is to find interaction between molecules and receive some biological benefit from this interaction. One of examples can be an interaction of thionine (tricyclic heteroaromatic molecule) with deoxyribonucleic acid (DNA). Moreover,... 

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