DNeasy tissue kit

Isolate genomic DNA from tissne/cells nsing DNeasy tissue kit (Qiagen) or phenol chloroform extraction. 

Cells are harvested and total DNA is extracted using DNeasy Tissue Kit. 

Genomic deoxyribonucleic acid (DNA) was extracted from cell lines with the DNeasy tissue kit (Qiagen, Valencia, CA). 

DNA was extracted from air-dried field collections or herbarium specimens with the DNeasy Plant Kit (Qiagen Inc., Valencia, California). One green shoot tip was used for extractions from large plants, or three to five green shoot tips from small plants. The extraction protocol provided by the manufacturer was used with the following modifications (provided by John Wheeler) (1) ground tissues were incubated in lysate buffer (kit Buffer API) 1 to 2 h and (2) two 50- il elutions (kit Buffer AE) were used in the final step, with each elution allowed to incubate 30 to 60 min. 

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