Deoxyribonucleic acid deoxyribonuclease

Deoxyribonuclease (DNAase), an enzyme that degrades deoxyribonucleic acid, has been used in patients with chronic bronchitis, and found to produce favorable responses presumably by degrading the DNA, contributed by cell nuclei, to inflammatory mucus (213). Lysozyme [9001 -63-2] hydrolyzes the mucopeptides of bacterial cell walls. Accordingly, it has been used as an antibacterial agent, usually in combination with standard antibiotics. Topical apphcations are also useful in the debridement of serious bums, cellulitis, and dermal ulceration. 

List of Abbreviations PCR, polymerase chain reaction RT-PCR, reverse transcription polymerase chain reaction DNA, deoxyribonucleic acid RNA, ribonucleic acid RNase, ribonuclease mRNA, messenger RNA GABAa, y-aminobutyric acid type A cRNA, copy RNA dNTPs, deoxy nucleoside triphosphates MMLV, Mouse Moloney murine leukemia vims RT, reverse transcriptase bp, base pair Tm, melting temperature DEPC, diethylpyrocarbonate OD, optical density mL, milliliter SA-PMPs, streptavidin paramagnetic particles dT, deoxy thymidine DTT, dithiothreitol DNase, deoxyribonuclease RNasin, ribonuclease inhibitor UV, ultraviolet TBE, Tris-borate, 1 mM EDTA EDTA, ethylenediaminetetraacetic acid Buffer RET, guanidium thiocyanate lysis buffer PBS, phosphate buffered saline NT2, Ntera 2 neural progenitor cells... 

The following reviews describe the molecular and physical properties of this broad class of enzymes that catalyze the endohydrolysis of deoxyribonucleic acids and ribonucleic acids. The class includes deoxyribonuclease II [EC 3.1.22.1], Aspergillus deoxyribonuclease Ki [EC 3.1.22.2], deoxyribonuclease V [EC 3.1.22.3], crossover junction endoribonuclease [EC 3.1.22.4], and deoxyribonuclease X [EC 3.1.22.5]. See also Deoxyribonucleases Restriction Enzymes Ribonucleases... 

Deoxyhemerythrin, oxygenation of 862 Deoxyhemoglobin 850, 851 Deoxy-D-mannooctulosonic acid (KDO) 180s Deoxyribonuclease 652 - 653 Deoxyribonucleic acid. See DNA 2-Deoxyribose 200s 1-Deoxyxylulose 5-phosphate 735, 736 Dephosphorylation, cascade 542 Depsides 23 Depsipeptide 414... 

During the past decade a previously accepted notion that the deoxyribonucleic acid (DNA)-deoxyribonuclease (DNase I) reaction runs a uniform course with a uniform specificity began to be seriously doubted. It is now realized that striking differences exist between the early and terminal stages of the same reaction. The observed differences are not limited solely to the rate of the reaction but include variations in endo-or exonucleolytic character, in the effect of the divalent cation, and, finally, in the specificity toward the bases adjacent to the bond that is cleaved. 

Dicyclohexylcarbodiimide Dihydroxy acetone phosphate Deoxyribonucleic acid Complementary DNA Deoxyribonuclease Dinitrophenol L-Dihydroxyphenylalanine 2,3-Diphosphoglycerate Ethylenediaminetetraacetic acid Eicosapentaenoic acid Endoplasmic reticulum Fatty add... 

B25. Brody, S., and Balis, M. E., Mechanism of growth. I. Interrelation between deoxyribonuclease and deoxyribonucleic acid synthesis in nonmalignant growth. Cancer Res. 19, 538-543 (1959). 

Steigleder, G. K., Rust, S., and Koch, H., Digestibility of deoxyribonucleic acid of the nucleus by deoxyribonuclease in skin altered by psoriasis. Arch. Klin. Expil. Dermatol. 221, 203-206 (1965). 

Enzjonic hydrolysis of 2 -deoxyribonucleic acid with 2 -deoxyribonuclease and phosphodiesterase yields the 6 -phosphates of 2 -deoxyadenosine, 2 -deoxyguanosine, 2 -deoxycytidine, and th5onidine. The ion-exchange, chromatographic behavior of these four 2 -deoxynucleotides resembles that of the 5 -phosphates of ribonucleosides, rather than that of 3 -phos-phate (or 2 3 -cyclic phosphate) derivatives. Additionally, it was found that 5 -nucleotidase (which hydrolyzes ribonucleoside 5 -phosphates but... 

Cytochrome 62 is also called yeast lactate dehydrogenase (EC 1.1.2.3, lactate cytochrome c oxidoreductase) (204 205]. This cytochrome possesses both FMN and protoheme (206-211). Although the native enzyme also contains deoxyribonucleic acid, this moiety can be removed by deoxyribonuclease without affecting the enzymic activity of the enzyme (212) and is supposed to be a heterogeneous component (213). This enzyme completely differs in its heme content from animal lactate dehydrogenase and also from yeast n(—)-lactate dehydrogenase. 

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