Deoxyribonucleic acid degraded

Deoxyribonuclease (DNAase), an enzyme that degrades deoxyribonucleic acid, has been used in patients with chronic bronchitis, and found to produce favorable responses presumably by degrading the DNA, contributed by cell nuclei, to inflammatory mucus (213). Lysozyme [9001 -63-2] hydrolyzes the mucopeptides of bacterial cell walls. Accordingly, it has been used as an antibacterial agent, usually in combination with standard antibiotics. Topical apphcations are also useful in the debridement of serious bums, cellulitis, and dermal ulceration. 

Dornase alfa (Pulmozyme ) is a recombinant human (rh) DNase that selectively cleaves extracellular deoxyribonucleic acid (DNA). This DNA is released during neutrophil degradation and contributes to the high viscosity of CF sputum. Nebulization of dornase alfa 2.5 mg once or twice daily improves daily pulmonary symptoms and function, reduces pulmonary exacerbations, and improves quality of life.16 N-acetylcysteine and hypertonic saline are other mucolytic agents that are occasionally used however, they are not preferred agents due to a greater incidence of bronchospasm and unpleasant odor and taste.5... 

Que BG, Downey KM, So AG (1980) Degradation of deoxyribonucleic acid by a 1,10-phenanthroline-copper complex the role of hydroxyl radicals. Biochemistry 19 5987-5991 Quintiliani M (1983) Cellular thiols and radiation response. In Balzani V (ed) Baxendale Memorial Symposium. Lo Scarabeo, Bologna, pp 81-108... 

Bleomycins (BLMs) (Figure 3a) are a family of glycopeptide-derived antibiotics that have the ability to bind and degrade deoxyribonucleic acid (DNA), which... 

More difficulty was encountered with the pyrimidine deoxynucleosides. It was tacitly assumed that the sugar radical in thymidine and deoxy-cytidine, by analogy with the ribonucleosides, is attached to position Nl. The first significant experiment111 to shed some light upon the validity of this assumption was based upon the methylation studies of Levene and Tipson.108 Deoxyribonucleic acid from thymus was methylated with dimethyl sulfate plus alkali, and the product was degraded by strong-acid hydrolysis. One of the products obtained was 3-methylthymine (XI).111 A... 

Jones AS, Mian AM, Walker RT. The alkaline degradation of deoxyribonucleic acid derivatives. J. Chem. Soc. Section C. 1968 2042. 

A preliminary chemical investigation of the polysaccharides of M. tuberculosis has recently been undertaken by W. N. Haworth and the authors. It was found that two stable and probably degraded polysaccharide fractions could be isolated from the defatted cells by the action of sodium hydroxide. Both products were serologically active at a dilution of 1 2,000,000 the first fraction ([a] D + 85° in water) was derived mainly from the somatic portion of the cell, while the second + 27° in water) was found in the ether-soluble lipid constituents. Both fractions were intimately associated with deoxyribonucleic acid, which was identified by nitrogen and phosphorus analyses, by the Dische test and by spectrophotometric measurements. 

Levinthal, C. Davison, P.F. Degradation of deoxyribonucleic acid under hydrodynamic shearing forces. J. Mol. Biol. 1961, 3, 674-683. 

The carbohydrate component of ribonucleic acid and, therefore, of the corresponding purine nucleosides was identified as a pentose by Hammar-sten and, later, as D-ribose by degradation and then by synthesis. Because of the instability of 2-deoxy-D-erythro-peutose ( 2-deoxy-D-ribose ), its isolation from deoxyribonucleic acid was much more difficult. Levene and coworkers finally obtained the crystalline sugar from deoxyguanosine by brief treatment with dilute mineral acid. They established its identity by comparison with synthetic 2-deoxy-D-threo-pentose and 2-deoxy-L-er /[Pg.303]

Virus, bacteria, bacterial spores, as well as toxins have been found to be degraded by MgO chlorine adducts. The bacterial outer membrane appears to be ruptured by the oxidizing action of the chlorine as well as the abrasive action of the MgO crystals. It also appears that the polar bacterial DNA (deoxyribonucleic acid) is strongly adsorbed by the MgO. 

Bernardi G., Champagne M., Sadron C. (1960). Enzymatic degradation of deoxyribonucleic acid into sub-units. Nature (London) 188 228-229. 

Levinthal C, Davison P F (1961). Degradation of deoxyribonucleic acid hydrodynamic shearing forces. J. Molec. Biol. 3 674-683. 

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