Deoxyribonuclease and

Lacks, S.A. (1970). Mutants of Diplococcus pneumoniae that lack deoxyribonucleases and other activities possibly pertinent to genetic transformation. J. Bacteriol. 101, 119-131. 

Part Three reviews representative multienzyme compositions, such as pancxeatin, as well as premising recent developments with glucocerebtosidaae, deoxyribonuclease and protein inhibitors of elastase. This part integrates biochemical, experimental, and clinical data of therapeutic enzymes, such as asparaginase, bromelain, hyaluronidase, and cysteine proteinsses, about which... 

The general scheme for the degradation of nucleic acids has much in common with that of proteins. Nucleotides are produced by hydrolysis of both dietary and endogenous nucleic acids. The endogenous (cellular) polynucleotides are broken down in lysosomes. DNA is not normally turned over rapidly, except after cell death and during DNA repair. RNA is turned over in much the same way as protein. The enzymes involved are the nucleases deoxyribonucleases and ribonucleases hydrolyze DNA and RNA, respectively, to oligonucleotides which can be further hydrolyzed (Fig. 15-18), so eventually purines and pyrimidines are formed. 

Keller and Cohen (36) also subjected to chromatography acidic extracts of cattle pancreatic microsomes and ribosomes. In microsomes they found the expected amounts of all enzymes which are known to be stable in acid, viz., chymotrypsinogen A, trypsinogen, deoxyribonuclease, and ribonuclease. The amounts of chymotrypsinogen B were abnormally low and ribonuclease B was perhaps not present. The results concerning ribosomes were made somewhat uncertain by the ability of these particles to incorporate proteins from the medium. Nevertheless, a series of characteristic enzymes could be isolated from what appears to be the very site of their biosynthesis. 

C. perfringens is the most important of the histotoxic clostridia that cause tissue infections in humans, especially of the muscle tissue (clostridial myonecrosis or gas gangrene). The organism is more aerotolerant than most other anerobes. In addition to toxins and enzymes, many of which have lethal, cell-destroying and hemolytic properties, a number of nonlethal enzymes are also produced and apparently contribute to the invasiveness of the organism in the tissue. These include collagenase, deoxyribonuclease, and hyaluronidase. 

B25. Brody, S., and Balis, M. E., Mechanism of growth. I. Interrelation between deoxyribonuclease and deoxyribonucleic acid synthesis in nonmalignant growth. Cancer Res. 19, 538-543 (1959). 

Enzjonic hydrolysis of 2 -deoxyribonucleic acid with 2 -deoxyribonuclease and phosphodiesterase yields the 6 -phosphates of 2 -deoxyadenosine, 2 -deoxyguanosine, 2 -deoxycytidine, and th5onidine. The ion-exchange, chromatographic behavior of these four 2 -deoxynucleotides resembles that of the 5 -phosphates of ribonucleosides, rather than that of 3 -phos-phate (or 2 3 -cyclic phosphate) derivatives. Additionally, it was found that 5 -nucleotidase (which hydrolyzes ribonucleoside 5 -phosphates but... 

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