Electrophoresis density gradient

Catsimpoolas N, Griffith AL, Skrabut EM, et al. 1976. Differential Cr uptake of human peripheral lymphocytes separated by density gradient electrophoresis. Cell Immunol 25 317-321. 

Mammalian Cells, Density Gradient Electrophoresis of (Tulp). 30 141... 

Cells in aqueous suspension preferably are separated by physical methods under well-defined experimental conditions rather than by chemical modification that influences cellular viability or initiates cellular processes. Moderate physical forces and short separation time gready contribute to cellular integrity. In this chapter, the application of density gradient electrophoresis to the separation of mammalian cells is described. This process may be defined as the differential zonal movement of cellular particles of anionic character suspended in a stabilized liquid column under the influence of an external electrical field so that collection of differentially moved zones is feasible. 

Two of the more critical steps in density gradient electrophoresis are undisturbed introduction of the (thin) sample layer into the column and the undisturbed fractionation of the column after electrophoresis. 

DENSITY GRADIENT ELECTROPHORESIS OF MAMMALIAN CELLS 159 D. THE LKB COLUMN... 

As prototypic mammalian cells, erythrocytes have been used almost solely to test the resolving power of density gradient electrophoresis columns. To perform a large number of tests, erythrocytes may be fixed by dilute aldehyde solutions (Vassar et al., 1972) to obtain particles that are chemi-... 

Platsoucas et al. (1980) subjected human faucal tonsil cells to density gradient electrophoresis. Low-mobility fractions were highly enriched with B cells and were contaminated only with 10—30% T cells. The high-mobility fractions were comprised of T cells with minimal contamination of B cells. Intermediate fractions contained approximately equal amounts of T and B lymphocytes. Human tonsillar lymphocytes, preenriched by E rosetting, showed a much more narrow migration profile but purification of T x, Ty, and Tot (IgA receptor) cells was not impressive, if achieved at all. 

Green, M. L., and Crutchfield, G. (1971). Density-gradient electrophoresis of native and of rennet-treated casein micelles. J. Dairy Res. 38, 151-164. 

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