Density gradient electrophoresis electrodes

Figure 10. Filling of the electrophoresis chamber. For a detailed description see text. Parts that contain liquid are depicted by shading, (a) At right, electrode compartments are filled with electrophoresis buffer. The space between membrane and middle plate is filled as described. At left, the separation chamber with its top and bottom cone is filled with density gradient, cell layer (dotted line), and overlay, (b) The top and bottom plates move to the left but the middle plate is stationary, (c) The electrode compartments are aligned with the hollow separation chamber in the middle plate, flushing of the electrodes is initiated, and electrophoresis started. In (b) and (c), in- and outflow tubes, cocks, and connectors plus springs are omitted for reasons of simplicity. After electrophoresis, top and bottom plates move to the right until the configuration is as in (a) and contents of the chamber are then fractionated via the top cone by pumping in cushion liquid via the bottom cone.

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