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Mammalian cells, density gradient electrophoresis

Mammalian Cells, Density Gradient Electrophoresis of (Tulp). 30 141... [Pg.255]

Cells in aqueous suspension preferably are separated by physical methods under well-defined experimental conditions rather than by chemical modification that influences cellular viability or initiates cellular processes. Moderate physical forces and short separation time gready contribute to cellular integrity. In this chapter, the application of density gradient electrophoresis to the separation of mammalian cells is described. This process may be defined as the differential zonal movement of cellular particles of anionic character suspended in a stabilized liquid column under the influence of an external electrical field so that collection of differentially moved zones is feasible. [Pg.142]

DENSITY GRADIENT ELECTROPHORESIS OF MAMMALIAN CELLS 159 D. THE LKB COLUMN... [Pg.159]

As prototypic mammalian cells, erythrocytes have been used almost solely to test the resolving power of density gradient electrophoresis columns. To perform a large number of tests, erythrocytes may be fixed by dilute aldehyde solutions (Vassar et al., 1972) to obtain particles that are chemi-... [Pg.177]

See other pages where Mammalian cells, density gradient electrophoresis is mentioned: [Pg.141]    [Pg.143]    [Pg.145]    [Pg.147]    [Pg.149]    [Pg.151]    [Pg.153]    [Pg.155]    [Pg.161]    [Pg.163]    [Pg.165]    [Pg.167]    [Pg.169]    [Pg.171]    [Pg.173]    [Pg.175]    [Pg.177]    [Pg.179]    [Pg.181]    [Pg.183]    [Pg.185]    [Pg.187]    [Pg.191]    [Pg.193]    [Pg.195]    [Pg.197]    [Pg.385]    [Pg.161]    [Pg.165]   
See also in sourсe #XX -- [ Pg.141 , Pg.142 , Pg.143 , Pg.144 , Pg.145 , Pg.146 , Pg.147 ]



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