Cytotoxicity Screen

From Table 4 it is evident that several natural and modified polyanions did not induce insulinoma cell detachment. Furthermore, the DNA content, a quantitative metric of cell viability, was in general near the baseline (100%) observed in the control studies with PBS. In generalboth the cell attachment and DNA indices gave similar indications of cytotoxicity. Therefore, in an effort to unify these measures the third column of Table 4 reports an overall ranking of the polymer 

Effect of Polymer Molecular Weight on Membrane Formation 

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George, S. et al. (2010) Use of a rapid cytotoxicity screening approach to engineer a safer zinc oxide nanoparticle through iron doping. ACS Nano, 4 (1), 15-29. 

Need to Coordinate Cytotoxicity Screening with Other Discovery Safety Assessments 327... 

In terms of SAR information, substituting the indole structure (azaindoles X or Y = N) yielded inactive or compounds of low cytotoxicity. The same holds true for N-alkylation or substitution of the methoxy-group in the indole core structure of (12a) vs. a methyl group. In summary, the results from the cellular cytotoxicity screening suggested the benefit of the 5-methoxy-indolyl-group for potent antitumour activity. 

U.S. government s war on cancer led to a massive screening program by the National Cancer Institute, which yielded important chemotherapeutic agents such as Taxol , vinblastine, and camp-tothecin. Moreover, the cytotoxicity screens developed remain the most common primary assay for evaluating crude natural product extracts. At the same time, natural products were heavily exploited outside the antimicrobial and anticancer areas. Two classic examples are the medicinal chemistry endeavors devoted to the total and semisynthesis of thousands of compounds based on opium alkaloids as analgesics and steroids as hormones. 

Moreira E, Cecy C, Nakashima T, Cavazzani JR, Miguel OG, Krambeck R (1980) Solasodine in Solanum erianthum D.Don. Tribuna Fannac 48 24-43 Moreno-Murillo B, Fajardo MVM, Suarez MM (2001) Cytotoxicity screening of some South American Solanaceae. Fitoterapia 72 680-685... 

High-Content Cytotoxicity Screening by iPSC-Derived Hepatocytes... 

Correche ER, Carrasco M, Giannini F et al (2002) Cytotoxic screening activity of secondary lichen metabolites. Acta Farm Bonaerense 21 273-278... 

Enhancement of Polymer Biocompatibility. The polymers as initially prepared were all found to be cytotoxic, most likely due to residual catalyst in the polymer. The extraction scheme was desiped to remove water and organic extractable materials. After following the extraction scheme, all of the polymers passed the cytotoxicity screen. 

The known phenanthroindohzidine alkaloids tyloindicines F (1285) and I (1286), isolated fixim the leaves of T. indica, were included in a cytotoxicity screen as part of the National Cancer Institute s in vitro program that uses a panel of 58 cell lines derived from major human tumors. ° At concentrations of 10 —10 M, the compounds produced LCsp-level responses against most lung cancer and melanoma cell lines, as well as some CNS cancer cell lines. 

Froscio, S. M. Fanok, S. Humpage, A. R. Cytotoxicity screening for the cyanobacterial toxin cylindrospermopsin. J. Toxicol. Environ. Health Part A 2009, 72, 345-349. 

Merrick, P Nieminen, A. L. Harper, R. A. Herman, B. Lemasters, J. J. Cytotoxicity screening of surfactant-based shampoos using a multiwell fluorescence scanner correlation with Draize eye scores. Toxicol. In Vitro 1996, 10, 101. 

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