Cytosol, isolation

WS-7528 [132147-69-4][VI] a nonsteroidal estrogen, is an isoflavone which has been isolated from Streptomjces sp. No. 7528 and is an estrogen agonist. It inhibits [3ff]-estradiol binding to its receptor in rat uterine cytosol at an inhibitor for 50% of the rats tested (IC q) concentration of 5.7 nM. It also induces the growth of estrogen-dependent human breast cancer cell line MCE-7 (7). 

The cytosolic dyneins bear many similarities to axonemal dynein. The protein isolated from C. elegans includes a heavy chain with a molecular mass of approximately 400 kD, as well as smaller peptides with molecular mass ranging from 53 kD to 74 kD. The protein possesses a microtubule-activated ATPase... 

Sirolimus (SRL), also termed rapamycin is a macrolide lactone isolated from the ascomycete species Stre-ptomyces hygroscopicus. After binding to its cytosolic receptor FKBP-12 the resulting complex inhibits the multifunctional serine-threonine kinase mTOR (mammalian target of rapamycin). Inhibition of mTOR prevents activation of the p70S6 kinase and successive... 

FIGURE 10.3 Structure of the BHTOH-KLH conjugate utilized for raising polyclonal antibodies. Western blots from 2D gels of cytosolic proteins isolated from mouse lung epithelial cells. The blot on the right is from cells incubated with BHT-QM and the blot on the left is from untreated cells. Immunoreactive proteins were identified by electrospray LC-MS analysis of the tryptic digests. Source From Ref. 39, with permission from the American Chemical Society. 

Proulx [30] summarized the published lipid compositions of BBM isolated from epithelial cells from pig, rabbit, mouse and rat small intestines. Table 3.1 shows the lipid make-up for the rat, averaged from five reported studies [30], On a molar basis, cholesterol accounts for about 50% of the total lipid content (37% on a weight basis). Thus, the cholesterol content in BBM is higher than that found in kidney epithelial (MDCK) and brain endothelial cells (Table 3.1). Slightly different BBM lipid distribution was reported by Alcorn et al. [31] here, the outer (luminal) leaflet of the BBM was seen to be rich in sphingomyelin content, while the inner leaflet (cytosol) was rich in PE and PC. Apical (brush border) and basolateral lipids are different in epithelia. The basolateral membrane content (not reported by... 

The enzyme involved in the degradation of the dyes has been shown to be azoreductase. The enzymes were first isolated from the intestinal microflora and was later found to be produced by the cytosolic and microsomal fractions of the liver [47]. The enzyme was sensitive to oxygen and was inactivated by oxygen. In experiments involving intestinal anaerobic bacteria, Rafii et al. found the requirement of... 

Estradiol. The first neuroactive steroid receptor type to be recognized was that for estradiol [3]. In vivo uptake of [3H] estradiol, and binding to cell nuclei isolated from hypothalamus, pituitary and other brain regions, revealed steroid specificity closely resembling that of the uterus, where steroid receptors were first discovered [3]. Cytosolic estrogen receptors isolated from pituitary and brain tissue closely resemble those found in uterus and mammary tissue. A hallmark of the estrogen receptor is its existence... 

Inoue Y, Sperelakis N 1991 Gestational change in Na+ and Ca2+ channel current densities in rat myometrial smooth muscle cells. Am J Physiol 260 C658-C663 Kamishima T, McCarron JG 1996 Depolarization-evoked increases in cytosolic calcium concentration in isolated smooth muscle cells in rat portal vein. J Physiol 492 61-74 Khan R, Mathroo-Ball B, Arulkumaran S, Ashford ML 2001 Potassium channels in the human myometrium. Exp Physiol 86 255-264... 

A cytosolic binding protein for CsA was first isolated in 1984 and named cyclophilin, later cyclophilin A (CyPA), in reference to its high affinity for CsA (18). CyPA is a basic, abundant protein with a mass of 18 kDa, and it is found in a variety of tissues. The first clue to its function came in 1989 when two independent groups isolated the enzyme that catalyzes pepti-dyl proline isomerization/peptidylprolyl cis-trans isomerase (EC 5.2.1.8 PPIase), in protein chains and (re)discovered CyPA (19, 20). CyPA is a potent PPIase, and its enzymatic activity is strongly inhibited by CsA. 

Breyer-Pfaff U, Nill K. Carbonyl reduction of naltrexone and dolasetron by oxidoreductases isolated from human liver cytosol. J Pharm Pharmacol 2004 56(12) 1601—1606. 

Soine PJ, Blanke RV, Schwartz CC. 1984b. Isolation of chlordecone binding proteins from pig liver cytosol. J Toxicol Environ Health 14(2-3) 305-317. 

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