Cytochemistry/Histochemistry

Plant cytochemistry/histochemistry continues to evolve as fluorescence microscopy (16-19), confocal fluorescence microscopy (20,21), and microspectrophotometry (22) expand our quantitative knowledge of the distributions of chemical constituents in plant cells and tissues. With regard to microspectrophotometry, this is possible for single cells, as the Arcturus Corporation (Mountain View, C A) has developed an instrument capable of isolating single cells. 

Preparation of Plant Cells and Tissues for Light Microscope Cytochemistry/Histochemistry... 

Maier, D.M. and Zaiman, H. (1966) The development of lysosomes in rat skeletal muscle in trichinous myositis. Journal of Histochemistry and Cytochemistry 14, 396-400. 

Yamashita S. Heat-induced antigen retrieval Mechanisms and application to histochemistry. Progress in Histochemistry and Cytochemistry 2007 41 141-200. 

The President of BioGenex, Dr. Kalra, invited three distinguished experts of IHC, Drs. Clive R. Taylor, Ronald A. DeLellis, and Hector Battifora to evaluate AR. They repeated this heat-induced AR protocol at their labs, and were all impressed by the great effects of this simple method. The first landmark article of AR was quickly accepted by Dr. Paul Anderson, Editor of the Journal of Histochemistry and Cytochemistry and published in 1991.5... 

Kevin A. Roth, Robert and Ruth Anderson Professor and Chair, Department of Pathology, University of Alabama at Birmingham, Director of Alabama Neuroscience Blueprint Core Center, and Editor-in-Chief of Journal of Histochemistry and Cytochemistry, Birmingham, AL... 

Karnovsky, M. and Roots, L.A. (1964). A direct colouring method for cholinesterases. Journal of Histochemistry and Cytochemistry 12 219-225. 

Although many published volumes exist regarding the cytochemical/ histochemical localizations of cellular and tissue chemicals for animal systems (1-10), there are only a few relatively recent monographs concerning plant cell/tissue cytochemistry and histochemistry (11-15). 

Gahan PB. Plant Histochemistry and Cytochemistry An Introduction, Academic Press, London, UK, 1984. 

Cordon-Cardo, C., O Brien, J.P., Boccia, J., Casals, D., Bertino, J.R. and Melamed, M.R. (1990) Expression of the multidrug resistance gene product (P-glycoprotein) in human normal and tumor tissues. Journal of Histochemistry and Cytochemistry, 38, 1277-1287. 

Glick, D. (1949). Techniques of Histochemistry and Cytochemistry. Interscience, New York. 

Glick, D. (1953). Approaches to quantitative histochemistry and cytochemistry. Intern. Rev. Cytol. 2, 447-474. 

Yamashita, S. Histochemistry and cytochemistry of nuclear receptors. Prog Histochem. Cytochem. 2001, 36(2), 91—176. 

Bioindicators. Water-soluble, colorless tetrazolium salts can be reduced to water-insoluble, deeply colored formazans. The reduction of tetrazolium salts in plant tissue at pH 7.2 was first demonstrated in 1941 [77], Tetrazolium salts have since been used in biochemistry, cytochemistry, and histochemistry because of the great sensitivity of this reaction. They can be used to detect biological redox systems in blood serum, in living cells, tissues, tumors, and bacteria. Tetrazolium Blue [167429-81-7] (48) is a particularly sensitive reagent. 

Vignery A, Niven-Fairchild T, Ingbar DH, Caplan M. Polarized distribution of Na +, K + -ATPase in giant cells elicited in vivo and in vitro. Journal of Histochemistry and Cytochemistry 1989, 37, 1265-1271. 

Pringle GA, Dodd CM. Immunoelectron microscopic localization of the core protein of decorin near the d and e bands of tendon collagen fibrils by use of monoclonal antibodies. Journal of Histochemistry and Cytochemistry 1990, 38, 1405-1411. 

Leiden University Medical School, Department of Histochemistry and Cytochemistry, Sylvius Laboratories, P.O. Box 722, Leiden 2405, The Netherlands. 

Wood G, et al. Suppression of Endogenous Avidin-Binding Activity in Tissues and Its Relevance to Biotin-Avidin Detection Systems. Journal of Histochemistry and Cytochemistry 2981 29 1196-204. 

Schionning JD, Poulsen EH, Moller-Madsen B, et al. 1991. Ultrastructural localization of mercury in rat dorsal root ganglia after exposure to mercury vapor. In Graumann W, ed. Progress in histochemistry and cytochemistry, vol. 23, No. 1-4 Histo-and cytochemistry as a tool in environmental toxicology. 

Immunohistochemical localization in normal tissues of different epitopes in the multidrug transport protein PI70 evidence for localization in brain capillaries and crossreactivity of one antibody with a muscle protein. The Journal of Histochemistry and Cytochemistry, 37, 159-164. 

Gao, B., Stieger, B., Noe, B., Fritschy, J.M., and Meier, P.J. (1999) Localization of the organic anion transporting polypeptide 2 (Oatp2) in capillary endothelium and choroid plexus epithelium of rat brain. The Journal of Histochemistry and Cytochemistry, 47, 1255-1264. 

Confocal scanning microspectrofluorometry reveals specific anthracycline accumulation in cytoplasmic organelles of multi-drug resistant cancer cells. Journal of Histochemistry Cytochemistry, 46 (12), 1369-1376. ). 

Schnitzer, J.J., et al. Absolute quantitative autoradiography of low concentrations of 1251-labeled proteins in arterial tissue. Journal of Histochemistry and Cytochemistry, 1987, 35(12), 1439-1450. 

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