Culture media selective

Eisenblatter T, Psathaki K, Nitz T et al. (2001) Cell Culture Media Selection and Standardization. Kobiler. Blood-Brain Barrier. Kluwer Academic/Plenum Publishers, New York... 

Polyhedra bioactivity can be compromised due to nutrient limitations, causing abnormalities in virion development and occlusion within polyhedra (Rollinson et al., 1965 Slavicek et al., 1995 Belloncik et al., 1997). The most important aspects of culture medium selection are pH, osmolar-ity, and organic salt components. Components such as amino acids, vitamins, and carbon source (glucose for example), are typically found in the basal culture medium formulation. However, these simple formulations cannot promote cell growth themselves, unless supplemented with animal serum, normally 5-20% (v/v) fetal bovine serum (FBS) (Schlaeger, 1996). 

Phorbol esters are described as being protein kinase C activators and should be selective inhibitors for caveolae uptake [1 pM Phorbol 12-myristate 13-acetate (PMA) for 30 minutes] (48,68). The inhibition occurs as quickly as five minutes after adding PMA to cell culture medium and is not reversible within five hours (MA104 cells). However, PMA has several other effects on several other pathways. An example of this is that it has been described to stimulate macropinocytosis and transcytosis (see the following). 

A recent review of the metabolites of L. maculans and L biglobosa produced in diverse culture conditions [19] emphasized that both species biosynthesize host-selective and non-selective phytotoxins. Importantly, it was shown that the composition of metabolite profiles of L. maculans depended on the composition of the culture medium. In a chemically defined liquid medium, isolates virulent on canola produced mainly sirodesmin PL (1), a non-host-selective phytotoxin, minor sirodesmins with one, three, or four sulfurs bridging the dioxopiperazine ring (sirodesmin H (3) [20], sirodesmin J (4) and K (5) [21]) and phomalirazine (6) (Fig. 9.1). The various sirodesmins 1-5 and phomalirazine (6) caused necrotic lesions of different intensities on leaves of both resistant and susceptible plants. Phomalide (7), the first host-selective phytotoxin isolated from virulent isolates of L. maculans, caused disease symptoms (necrotic, chlorotic, and reddish lesions) on canola (susceptible to L maculans) but not on brown mustard or white mustard... 

Dilute cells in culture medium (without selection antibiotics) to 3.25 X10 cells/ml. 

Sterilization After a suitable culture medium is selected for the cultivation of a specific microorganism, it is poured into a culture vessel. If you use test tubes or flasks as your culture vessel, the ends of test tubes or flasks should be covered with a suitable closure to allow for the exchange of gases with the atmosphere, yet to keep foreign organisms out of the media. Various types of closures are used in the modern laboratory including cotton plugs, plastic foam, screw caps, metal caps, and aluminum foil. 

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