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Cross-linking dialysis

The cross-linking method relies on bifimctional reagents to form intermolecular linkages between the enzyme molecules to render them insoluble. Often albumin is added as an extender and glutaraldehyde is most commonly employed. This material can then be either formed as a free standing membrane or applied to the inner surface of the dialysis membrane... [Pg.63]

Variations to the slow-equilibration method include cross-linking of crystals prior to transfer into cryobuffers (Lusty, 1999), transfer of crystals into cryobuffer by dialysis, or the introduction of the cryobuffer during crystallogenesis. [Pg.60]

Purify the thiolated protein from excess DTT by dialysis or gel filtration using 50 mM sodium phosphate, 0.15 M NaCl, 1 mM EDTA, pH 7.2. The modified protein should be used immediately in a conjugation reaction to prevent sulfhydryl oxidation and formation of disulfide cross-links. [Pg.86]

Purify the conjugate by dialysis or gel filtration using a buffer suitable for the nature of the proteins being cross-linked. [Pg.206]

Dialyze the phycobiliprotein into 50 mM sodium borate, 0.3 M NaCl, pH 8.5 (note commercial preparations of these proteins come as an ammonium sulfate suspension). After dialysis, adjust the protein solution to a concentration of 1 mg/ml. Higher protein concentrations may be used, but the amount of cross-linking reagent added to each milliliter of the reaction should be proportionally scaled up, as well. Protect the protein solution from undue exposure to light. [Pg.384]

To simultaneously recover the metal and sulfuric acid from spent process liquors of nickel electrolysis, Xu and Yang [95] tested diffusion dialysis successfully. The membrane used was surface-cross-linked with aqueous ammonium to decrease waste volume expansion caused by the water osmosis. They could control nickel leakage within 4% and recover about 70% of the add. [Pg.322]

Xu, T.W. and Yang, W.H. (2004) Tuning the diffusion dialysis performance by surface cross-linking of PPO anion exchange membranes - simultaneous recovery of sulfuric add and nickel from electrolysis spent liquor of relatively low acid concentration. J. Hazardous Mater., 109 (1-3), 157-164. [Pg.348]

The polysilane core part within the shell cross-linked micelles was photodegraded by UV irradiation (>280nm) and dialysis against water produced nanometer-sized... [Pg.269]

The effect of copper deficiency is not fully understood. The element is required for lysil oxidase activity which is necessary for cross-linking of collagen. Its deficiency has heen associated with growth retardation and anemia. Also has in dialysis patients a correlation heen demonstrated between serum copper levels and superoxide dismutase activity. [Pg.889]

Other methods for the immobilization of whole cells are fixation on solid supports or matrices by adhesion or adsorption, e.g., on polyurethane foam, direct cross-linking e.g., by glutaraldehyde, or retention of the cells by hollow fibers. The simplest example of the last method is the separation of cells or enzymes from the assay by means of a dialysis lube which allows the exchange of the small substrate and product molecules but not of the larger components. This technique has been highly developed to provide the so-called membrane reactor for the fixation of enzymes. If charged ultrafiltration membranes are used, the cofactor can be retained in its native form316. [Pg.847]


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See also in sourсe #XX -- [ Pg.58 ]




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