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Core-like particles

Martyanov, I.N. Klabunde, K.J. Decomposition of CC13F over vanadium oxides and [MgVxOy]MgO shell/core-like particles. J. Catal. 2004, 224, 340-346. [Pg.60]

Viral structural proteins expressed by baculovirus infected insect cells assemble into multimeric structures that resemble viral core-like particles and virus-like particles (CLPs and VLPs, respectively). This presentation has brought the attention of researchers for the potential use of these structures as safe immunological reagents for virus or antibody detection in enzyme immuno assays, as vaccines, and more recently, as gene delivering systems for gene therapy [9]. [Pg.185]

French TJ, Roy P (1990), Synthesis of bluetongue virus (BTV) core-like particles by a recombinant baculovirus expressing the two major structural core proteins of BTV, J. Virol. 64 1530-1536. [Pg.456]

Tellinghuisen, T. L., Perera, R., and Kuhn, R. J. (2001). In vitro assembly of Sindbis virus core-like particles from cross-linked dimers of truncated and mutant capsid proteins./. Virol. 75, 2810-2817. [Pg.376]

Cleavage structural rearrangements, on influenza HAo, 329-331 CLPs. See Core-like particles (CLPs)... [Pg.533]

Hgure 10 Reconstructions of Blue tongue virus (BTV) and core-like particle obtained from frozen-hydrated specimens. (A) The surface representation of the outer shell of the reconstructed virus particle viewed along a threefold axis. The globular regions (VPS) are colored yellow and the sail-shaped spikes (VP2) are blue. Bars = 10 nm. (Reproduced from Hewat EA, Booth TF, and Roy P (1992) Journal of Structural Biology 109 61-69.) (B) The surface representation of the BTV core-like particle viewed down a threefold axis from outside the particle. Bar=nm. (Modified from Hewat EA, Booth TF, Loudon PT, and Roy P (1992) Virology 189 10-20.)... [Pg.3124]

Xu, P., Miller, S. Joklik, W. K. Generation of reovirus core-like particles in cells infected with hybrid vaccinia viruses that express genome segments LI, L2, L3, and S2. Virology 197, 726-731 (1993). [Pg.378]

Similar results were obtained from reconstitution experiments with DNA and a non-cross-linked octamer (Thomas and Butler, 1978). Nucleosome-like particles were observed in the EM and a pattern of histone cross-linking comparable to that of native chromatin was obtained. However, only 140-base-pair repeats were obtained upon micrococcal nuclease digestion instead of 200-base-pair repeats obtained for native rat liver chromatin (Noll and Komberg, 1977). This indicates that, in the absence of HI, only core particles can be reconstituted. Nevertheless, these studies with both cross-linked and reassembled un-cross-linked histones demonstrate that the octamer is a complete biological functional unit retaining the information for folding the DNA around the histone core. [Pg.15]

Production of Core and Virus-Like Particles with Baculovirus Infected Insect Cells... [Pg.183]

In this paper the fundamental aspects of process development for the production of core and virus-like particles with baculovirus infected insect cells are reviewed. The issues addressed include particle formation and monomer composition, chemical and physical conditions for optimal cell growth, baculovirus replication and product expression, multiplicity of infection strategy, and scale-up of the process. Study of the differences in the metabolic requirements of infected and non-infected cells is necessary for high cell density processes. In the bioreactor, the specific oxygen uptake rate (OURsp) plays a central role in process scale-up, leading to the specification of the bioreactor operational parameters. Shear stress can also be an important variable for bioreactor operation due to its influence on cell growth and product expression. [Pg.183]


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See also in sourсe #XX -- [ Pg.444 ]




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