Containment biological safety cabinets

Safety Equipment Enclosed containers, biological safety cabinets, and personal protection equipment are the main kinds of safety equipment. The primary roles are containment and barriers. Safety containers prevent the release of unsafe substances during normal activities and operations. An example is a safety centrifuge cup. 

Centers for Disease Control and Prevention and National Institutes of Health. Primary Containment of Biohazards Selection, Installation and Use of Biological Safety Cabinets. Washington, DC U.S. Department of Health and Human Services, 1995 (available on http // www.niehs.nih.gov/odhsb/biosafe/bsc/bsc.htm)... 

General handling philosophy Open handling of product not permitted. Keep containers closed. Avoid open handling on the bench. Use fume hoods, biological safety cabinet (BSC), or other ventilated control device for all aerosol generating activities... 

The facility must be designed to control access. Signs should designate restricted areas. Clothing requirements for entry should be identified. There should be directional air control with adequate ventilation rates. Primary containment equipment such as chemical fume hoods, a Class I or Class II (Type B) biological safety cabinet, or a glove box system should be available for use with known or suspect chemical carcinogens or other toxic substances. 

N o special containment devices or equipment such as a biological safety cabinet are usually required for manipulations of agents assigned to biosafety level 1. 

All manipulations involving infectious materials are to be conducted in biological safety cabinets orotherphysical containment devices within the containment module.No work in open vessels is to be conducted on the open bench. 

Respiratory protection is worn when aerosols cannot be safely contained (i.e., outside of a biological safety cabinet), and in rooms containing infected animals. 

All procedures within the facility with agents assigned to biosafety level 4 are to be conducted in C lass 111 biological safety cabinets orin Class 11 biological safety cabinets used in conjunction with one-piece positive pressure personnel suits ventilated by a life support system. Work with vital agents that require biosafety level 4 secondary containment capabilities can be conducted in class 11 biological safety cabinets within the facility without the one-piece positive pressure personnel suit if ... 

Contain in biological safety cabinets operations that generate aerosols. 

During the preparation of hazardous drugs, use a ventilated cabinet to reduce the potential for occupational exposure. Performance test methods and criteria for BSCs may be found in Primary Containment for Biohazards Selection, Installation and Use of Biological Safety Cabinets, second edition, CDC/NIH, 2000. A current field certification label should be prominently displayed on the ventilated cabinet per NSF/ANSI49. 

Biological Safety Cabinets Describes biological safety cabinets and methods for containment used in handling infectious biological microorganisms in the laboratory. 

FIGURE 7.3.4.3 Canopy Unit Exhaust for Class II Type AI BSC. The canopy unit (sometimes called the thimble) is designed to fit over the exhaust of the BSC but not tightly. There is a gap of about 1 inch between the canopy and the BSC exhaust so that air is also exhausted from the room. Canopy units are often used in small lab rooms where some exhaust ventilation is needed to remove chemical contaminants that might be in the air. (From Figure 4 in CDC/NIH, Primary Containment for Biohazards Selection, Installation, and Use of Biological Safety Cabinets, 3rd edition, CDC/NIH, Washington, DC, 2007 available at http //www.cdc.gov/od/ohs/biosfty/primary containment for biohazards.pdf.)... 

Biological Safety Cabinets or other physical containment devices used for all manipulations of agents that cause splashes or aerosols of infectious materials... 

Maximum containment equipment (i.e.. Class II Biological Safety Cabinet or partial containment equipment in combination with full body, air-supplied positive-pressure suit) used for all procedures and activities... 

From Figure 1 in CDC/NIH, Primary Containment for Biohazards Selection, Installation, and use cf Biological Safety Cabinets, 2009 http //www.cdc.gov/biosafety/pubUcations/bmbl5/BMBL5 appendixA. pdf, (accessed Dec 2014). 

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