Confocal microscopy imaging

Masters, B. R., and So, P. T. C. 1999. Multiphoton excitation microscopy and confocal microscopy imaging of in vivo human skin A comparison. Microsc. Microanal. 5 282-89. 

Fig. 21 Structures of hydrophilic 2PA fluorophores 49 used for cell staining, and confocal microscopy images of live NT2 cells incubated with a two-photon absorbing hydrophilic probe 49 and two-photon induced fluorescence image of fixed NT2 cells (60 x oil) stained with the hydrophilic probe 49...

Fig. 39 Aggregation of colloids coated with long DNA strands above a sticky surface leads to flying carpets, floating 2D crystals, (a) Confocal microscopy image of a flying carpet (scale bar is 10 pm), (b) Pair correlation function of the structure depicted in (a). The observed peaks match those expected for a perfect hexagonal crystal (black triangles). Reproduced with permission from [165]...

Fluorescent labelling of protein/RNA or DNA combined with optical or confocal microscopy imaging is a powerful widely used imaging technique that can (like Raman spectroscopy) also be applied to live cells or tissues. This technique, however, is limited by the number of fluorescent molecular... 

M 32] [P 30] The mixing process was visualized by epifluorescence and confocal microscopy images (see Figure 1.68) [112]. A bright inlet flow, labeled with a... 

A confocal microscopy image (Figure 19.1) evidences the 3D structure of hepatocytes after days of culture on a PEEK-WC-PU membrane cells organize in small aggregates, which would lead to better functional maintenance, maintaining a polygonal shape, so many of the features of the liver in vivo are reconstituted. 

This work was supported by the New Zealand Foundation for Research, Science and Technology. Thanks are due to W. A. Forster and R. E. Gaskin for constructive advice, Z. Q. Liu for confocal microscopy images, and M. Haslett for assistance with the preparation of this manuscript... 

Figure 14.5 (a-b) SEM images of stripe patterns widi 4 p and 0.6 p width, respectively, realized by aligning 200-nm-wide triangular nanoprisms by e-beam lithography (c-d) Confocal microscopy images of the NC / PMMA blend film deposited onto the same arrays. 

Figure 14.6 Confocal microscopy image collected on colloidal quantum dots deposited on a metallic nanopattem reproducing the acronym of our laboratory.

Fig. 8.8 Optical images (at an active zone I) of isolated silver clusters electrodeposited onto ITO by means of the double-pulse method ( i = —1,550 mV 2 = —VOO mV, 25 s) [37] (a) Scanning confocal microscopy image (topography mode), (b) Raman/fluorescence image of the same sample area, (c) SEM image corresponding to (a) and (b)...

Fig. 4.7 Fluorescence confocal microscopy images of the systems TM1 in contact with acetonitrile and in contact with Ca2+ (10 4 M, acetonitrile) (left). Profiles of cross sections of the images of TM1 before (a) and after (b) addition of Ca2+ (right).33 Reprinted with permission from35. Copyright 2004 American Chemical Society...

Fig. 11.2-7 Laser scanning confocal microscopy image of (a) Mutant Escherichia coli that does not bind to polymer 17. (b) A fluorescent bacterial aggregate due to multivalent interactions between the mannose-binding bacterial pili and the polymer 17 (superimposed fluorescence and...

Figure 7 Confocal microscopy images of FITC-insulin loaded microspheres. (Left) Msp A (right) Msp B. (From Ref 49 with permission.)...

Figure 9.2 is a schematic representation of CdSe QDs dispersed in poly(hexyl methacrylate) by in situ polymerization. The polymer with long alkyl branches is expected to prevent or reduce phase separation of the QDs from the polymer matrix during polymerization. This technique resulted in the preparation of a series of QD-based nanocomposite materials for which laser scanned confocal microscopy imaging revealed a nearly uniform dispersion of nanoparticles within the polymethacrylate matrix (Fig. 9.3). Notably, the resulting macroscopic QD-polymer composites appeared to be clear and uniformly colored.

Fig. 2 Scanning confocal microscopy images of blend films of ultra-high-molecular-weight polyethylene and 10 wt% EHO-OPPE (see Fig. 1). (a) As prepared film, (b) Uniaxially deformed sample drawn to a draw ratio of 80. Both images were acquired by detecting the polarization direction oriented parallel to the (eventual) deformation direction. Inset shows images acquired by detecting the orthogonal polarization direction. Adapted with permission from [35]. Copyright 2000 American Chemical Society...

In Fig. 5.17 a time series of confocal microscopy images is presented of a sample with a volume fraction of silica spheres of 0.025 and a volume fraction of boehmite rods of 0.0025. In the confocal microscopy images it is seen that with time the silica number density increases by sedimentation. Locally ordered structures are formed but no signs of depletion-induced phase transitions are found. 

Fig. 5.17 Confocal microscopy images of fluorescently labelled silica spheres (ui = 740 nm) mixed with boehmite rods of length L = 230 nm and diameter Z> = 10 nm various times T after mixing at 0.25 wt% rods and 2.5 wt% spheres. Images are 50 x 50 pm. Pictures reproduced from [38], Copyright 1999, by permission of the Royal Society of Chemistry...

The nanotubes were visualized by AFM, scanning tunneling microscopy (STM), and confocal microscopy images. ... 

Fig. 10 Intensity profile of a confocal microscopy image (left) of the Platinum(II) complex (right) in human dermal fibroblasts. ... 

Figure S. Confocal microscopy images of brain tumor cells grown in 3D matrices of gelatin-cellulose nanocomposites from wood fibers. Left panel shows the white light phase image of the 3D matrix with nanocomposite fibers and cells visible. Right panel shows fluorescent image of the same field with cells stained with the vital dye calcein. Scale bar indicates 20.55 microns.

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