Confocal fluorescence image

Figure 2. a) Wide field fluorescence microphotographs of a single CrAPO-5 crystal taken during heating b,c) confocal fluorescence images (top and side respectively) of the CrAPO-5 crystal at 660 K. Detection channel of 575-635 nm are represented in gray scale. 

Figure 5. a) Fluorescence microphotographs of ZSM-5 crystals taken during template removal and b) confocal fluorescence images taken at 700 K (561-nm laser, detection at 575-635 nm, intensity presented as a gray scale). 

Fig. 17. Confocal fluorescence imaging of [Zn(ATSM)] in IGROV cells (100 pM, where Q1 — Q2 = Me, M = Zn(II), R1 = R3 = H and R2 = R4 = Me, /ex = 488 nm, DMEM with 1% DMSO). Brightfield image shows formation of needle-like crystalline material on the cell plate (N.B. Small crystallites may be endocytosed by the cells rather than passively diffuse through the cell membrane).

Fig. 19. Typical bright-field and confocal fluorescence images of the glucose-ATSM Zn(II) derivative in cancer cells (/.ex = 360 nm, Aem = 530 nm). Diagrams adapted from Ref. (102).

Fig. 21. Molecular structures of new aromatic [M(ATSM)] analogs (a) M — Zn(II) and (b) M = Cu(II), (c) cytotoxicity tests in MCF-7 cells for the Zn(II) complex (group 2) and Cu(II) complex (group 3) and comparison with control and with cis-platin over a range of concentrations, (d) cell uptake profile monitored over 90 min, (e) confocal fluorescence imaging of Zn(II) complex in MCF-7 cells, at 100 pM cone, in DMEM, 1% DMSO (112,113).

Figure 14. Confocal fluorescence images of single molecules of (a) the model compound 69 and (c) the multichromophoric dendri-mer 70 in a polyvinylbutyral (PVB) film. The fluorescence intensity transients (fluorescence intensity versus time) exhibit typical on-off behavior for 69 (b) and jumps between different emissive levels for 70 (d). Pictures taken from ref. [48],...

Fig. 3 (a) Normalized excitation and emission spectra of 5-(and-6)-carboxy-fluorescein, succini-midyl ester, rhodamine 6G (R6G), and 6-carboxy-X-rhodamine dyes in pH 7.4 phosphate buffer, (b) Confocal fluorescence image of a mixture of five types of microsphere-DDSN complexes under 488-nm Argon-ion laser excitation. Reproduced with permission from Ref. [12]... 

Hazani M, Naaman R, Hennrich F, Kappes MM (2003) Confocal fluorescence imaging of DNA-functionalized carbon nanotubes. Nano Lett. 3 153-155. 

Fig. 3. Comparisons of wide-field (A) and confocal fluorescence images (B, mesoglea level C, apical) of rhodamine phalloidin-stained F-actin in a whole-mount hydra tentacle. The hydra was fixed and stained as described in Chapter 18. The bar represents 25 pm. All images were collected with a Nikon (New York) Microphot FX microscope (x40 objective lens). Confocal images were collected with the microscope connected to a Bio-Rad (Hercules, CA) MRC600 laser-scanning confocal system.

FIGURE 4.6 Quantification of fluorescence intensity (expressed in arbitrary units per square micron) of the confocal fluorescence images of SW-480 cells after incubation with various polysaccharide containing HPMA copolymer-FITC conjugates and the control polymer (P-F) after 24 h at 37°C. (From David, A. et al., Pharm. Res., 19, 1114, 2002. With permission.)... 

Leeson, D., Meyers, C., Subramanyan, K., and Hawkins, S., In vivo confocal fluorescence imaging of skin surface cellular morphology. Poster presentation at the AAD annual meeting, Washington, DC, February 2004. 

Figure 3 Confocal fluorescence images obtained following (A) passive diffusion of calcein for 4 hr, and (B) passive diffusion of Nile red for 4 hr. The optical section was obtained nominally at 10 pm below the skin surface. Magnification is 40x.

Fig. 6.7 Confocal fluorescence images of polymer microsphere uptake by mouse macrophages (P388D1) after 24h of incubation at 37°C in medium containing serum. The microspheres con-...

This technique also has important applications in medicine. For example, it can be used for the spectral classification of a normal human liver cell versus a cancerous liver cell as shown in Figure 4.14. Both the normal human liver cell (E) and the cancerous liver cell (F) contain three dominant types of spectra, each of which is displayed as a distinct colour. However, when the two cells are compared quantitatively, as shown in the histogram area measurements, the abnormalities in the cancerous cell can be quantified objectively. Another example of the use of fluorescence microscopy in medicine is illustrated in Figure 4.15. Using confocal fluorescence imaging, the uptake and distribution of drug (in this case an anthracycline) can be profiled in tumour cells. 

Figure 4.15 Confocal fluorescence imaging showing drug uptake and distribution in tumour cells (Reproduced, with permission, from Belhoussine, R., Morjani, H., Millot, J.M. etal.

Fig. 20. Oligosaccharide-mediated nuclear transport of glyco-QDs. (A) Chemical structures of neoglycoconjugates. (B) Synthesis of glyco-QDs by thiol exchange reaction. (C) Confocal fluorescence images (top panels) and differential interference contact (lower panels) of digitonin-permeabilized HeLa cells incubated with glyco-QDs. (D) Digitalized fluorescence intensity of different QDs in the nucleus. Adapted from Ref 204. (See Color Plate 42.)...

Figure 5.19 TEM images of pristine MWCNTs (a), one polymer layer-clicked WMCNT (b), two polymer layer-clicked MWCNT (c) and three polymer layer-clicked MWCNT (d). (e) Fluorescence microscopy image of RhB-clicked MWCNTs ( ex = 520-550 nm), and (0 confocal fluorescence image of singular tube of RhB-functionalized MWCNT (Xqx=543 nm). Reprinted with permission from Zhang et al ...

Figure 5.25 Representative TEM image (a) and SEM image (b scale bar 500 nm) of HPG-functionalized MWCNT and confocal fluorescence image of RhB-modified HPG-functionalized MWCNT (c). Reprinted with permission from Zhou et al...

Figure 3.21 Confocal fluorescence images of EGFP transfected HeLa cells using PEI-1.2k (a), GO-PEI-1.2k (b), at varying N/P ratios from 10 to 80. Scale bar = 50 rm. Reprinted with permission from ref. 184. Copyright 2011 The Royal Society of Chemistry.

A typical confocal fluorescence image of the Au-P30T blended film is shown in Figure 11.7. Randomly distributed circular features with sharp intensity contrast are present throughout the film, indicating distinct phases. The circular features are essentially a signature of the... 

Figure 11.3 Confocal fluorescence images of human leukocytes incubated with 50 nM fluorescein-labeled peptide fNLFNTK (a) and 50 nM 4 (b). Adapted with permission from Ref. [48]. Copyright 2004, American Chemical Society.

Figure 11.4 Confocal fluorescence images of J = overlay of H and I. Adapted from Ref. [63] HeLa cells incubated with column H = DNA with permission of CNRS and the Royal Society...

Figure 11.5 Confocal fluorescence images of A2780 cells incubated with complex 17 for 2 h (a) and for 72 h (b). Adapted from Ref. [65].

Figure 11.7 Confocal fluorescence images of PPC-1 cells incubated for 60 min with 50 (iM 19. (a) (in blue, iem = 465-495 nm) indicates the incorporation of 19 into the cellular cytosol. (b) (in green, iem > 660 nm) was collected...

Figure 11.15 (a) Confocal fluorescence images of human dermal fibroblast cells (HDF), human melanoma cells (C8161), and Chinese hamster ovary cells (CHO), incubated with 3 (100 (iM, 5 min, Aex=488 nm, scale bar = 10 (im). (b) A two-photon (TPE) luminescence image of live CHO cells incubated with 3 (,1 x = 758 nm, 180 fc excitation, scale bar... 

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